YAKHAK HOEJI (약학회지)
- Volume 40 Issue 5
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- Pages.558-562
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- 1996
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Isolation and Quantitative Analysis of Betulinic Acid and Alphitolic Acid from Zyziphi Fructus
대추로부터 베튜리닉 산과 알피톨릭 산의 분리 및 정량
- Bae, Gi-Hwan (College of pharmacy, Chungnam National University) ;
- Lee, Sang-Myeong (College of pharmacy, Chungnam National University) ;
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Lee, Eun-Sil
(College of pharmacy, Chungnam National University)
;
- Lee, Jun-Seong (College of pharmacy, Chungnam National University) ;
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Gang, Jong-Seong
(College of pharmacy, Chungnam National University)
- Published : 1996.10.01
Abstract
Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase column (Nova-Pak C18) with 0.05M Na2HPO4-methanol (19:81) in HPLC and detected at 210nm. Separation on HPTLC precoated silica gel F254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with p-anisaldehyde and detected at 540nm. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.