한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권1호
  • /
  • Pages.72-76
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

새로운 Baculovirus 전이벡터를 이용한 Escherichia coli ${\beta}$-galactosidase 유전자의 발현

Expression of Escherichia coli ${\beta}$-galactosidase Gene by New Transfer Vector of Baculovirus

  • 우수동 (서울대학교 농업생명과학대학 농생물학과) ;
  • 김우진 (서울대학교 농업생명과학대학 농생물학과) ;
  • 김혜성 (서울대학교 농업생명과학대학 농생물학과) ;
  • 진병래 (서울대학교 농업생명과학대학 농생물학과) ;
  • 강석권 (서울대학교 농업생명과학대학 농생물학과)
  • Woo, Soo-Dong (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
  • Kim, Woo-Jin (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
  • Kim, Hye-Seong (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
  • Jin, Byung-Rae (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
  • Kang, Seok-Kwon (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University)
  • 발행 : 1996.02.01
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초록

To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells and larvae of silkworm, Bombyx mori. The recombinant virus containing lacZ gene was isolated from BmN-4 cells coinfected with transfer vectro pBmKSK1-LacZ and wild type BmNPV genome, and analysed by Southern blotting. The expression of ${\beta}$-galactosidase was characterized by SDS-PAGE, Western blotting and ${\beta}$-galactosidase activity assay. The results showed that the level of expression in silkworm larvae was higher than that of BmN-4 cells.

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