한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제24권1호
- /
- Pages.67-71
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- 1996
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- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
Peptide Synthetase의 활성 Adenylate 형성 Domain의 발현
Expression of an Active Adenylate Forming Domain of Peptide Synthetase
- 김연옥 (원광대학교 자연과학대학 화학과) ;
-
김기영
(원광대학교 의과대학 병리학실)
;
-
이성
(한서대학교 식품 생물공학과)
;
-
이영행
(원광대학교 자연과학대학 화학과)
;
-
유병수
(원광대학교 자연과학대학 화학과)
- Kim, Yoen-Ok (Department of Chemistry, Wonkwang University) ;
-
Kim, Ki-Young
(Department of Medicine, Wonkwang University)
;
-
Lee, Seong
(Department of Food and Biotechnology, Hanseo University)
;
-
Lee, Young-Haeng
(Department of Chemistry, Wonkwang University)
;
-
Yu, Byung-Soo
(Department of Chemistry, Wonkwang University)
- 발행 : 1996.02.01
초록
The plasmid pK8 was constructed to verify the existence of an adenylate domain in peptide synthetase by using pGC12. 1.2 kb fragment, coding tyrocidine synthetase 1 (123 kDa) was deleted, and 79.6 kDa one was expressed in Escherichia coli XL1-blue. The truncated multienzyme activated phenylalanine and substrate analogues with comparable kinetics as the over expressed synthetase. ATP-[