대한바이러스학회지 (The Journal of Korean Society of Virology)

대한바이러스학회 (The Korean Society of Virology)
권호 표지

소 허피스바이러스 gIII 유전자 크론닝 및 발현

Cloning and Expression of Bovine Herpesvirus-1 gIII of Korean Isolate PQ Strain

  • 권창희 (수의과학연구소, 테네시대학 미생물학교실) ;
  • 민부기 (수의과학연구소, 테네시대학 미생물학교실)
  • Kweon, Chang-Hee (National Veterinary Research Institute, Department of Microbiology, University of Tennese) ;
  • Min, Boo-Ki (National Veterinary Research Institute, Department of Microbiology, University of Tennese)
  • 발행 : 1996.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The gene encoding gIII of bovine herpesvirus type 1 (BHV-1) PQ strain was cloned and expressed in baculovirus. Although the gIII gene is located in Hind III I fragment as the case of the other BHV-1 strains, differences in size and restriction endonuclease site within the fragment were identified. The gIII expression was predominantly detected on the surface on insect cells by indirect immunofluoresecnce assay using monoclonal antibody. The western blotting analysis also revealed the presence of expressed protein of a similar molecular size to the original gIII protein. The immunogenicity of expressed protein were tested in guinea pigs. The immunized guinea pigs with expressed protein developed the neutralizing antibodies against BHV-1.

키워드