Effects of Stearic, Oleic and Elaidic Acid on Cellular Lipids and Their Fatty Acid Composition in Hep-$G_2$ Cells

단일지방산 첨가에 의한 간세포의 지질조성과 지방산조성에 미치는 영향

  • 김대진 (동아대학교 생명자원과학부) ;
  • 조병희 (미국일리노이대학교 영양학부)
  • Published : 1996.06.01


The effects of stearic(18 : 0, SA), oleic(18 : 1 cis, OA) and elaidic acid(18 : 1 trans, EA) on the cell growth, contents of cellular lipids, and the fatty acid composition of cellular and medium lipids in Hep-G$_2$cells were evaluated. The cells were incubated in serum-free medium containing 25, 50, 100 and 200$\mu$M of a fatty acid combined with albumin for 2 days. The fatty acid concentration up to 100$\mu$M showed the normal growth, but the cell growth decreased in the presence of 200$\mu$M fatty acid. The treatment of cells with 100$\mu$M of a fatty acid for two days significantly(p<0.05) increased the cellular triglyceride(TG) content in all fatty acid groups compared to control, but TG contents was not significantly different among all treatment group, but total cholesterol(TC) was the highest level in EA group. The level of free cholesterol(FC) and cholesteryl ester(CE) was similar to those of TC in all fatty acid treated group. The cellular phospholipid(PL) contents were similar between the control and all fatty acid groups. The treatment of cells with SA has no notable effects on the fatty acid composition of TG, CE and PL. The OA treatment caused significant increases in CE(51.2%) and PL(29.8%), but not in TG. The EA treatment resulted in 10.1, 10.7 and 7.8% of $C_{18:1\;trans}$ content in cellular TG, CE, and PL. The TG, CE and PL of medium were relatively similar between SA and OA groups. In EA treated group, TG, CE and PL of medium contained 17.0%, 0.7% and 5.6% of $C_{18:1\;trans}$, respectively.



  1. Metabolism v.14 Serum cholesterol response to changes in the diet, IV. Particular saturated fatty acids in the diet Keys, A.;Anderson, J. T.;Gronde, F.
  2. Am. J. Clin. Nutr. v.17 Quantitative effect of dietary fat on serum cholesterol in man Hegested, D. M.;McGandy, R. B.;Myers, M. L.;Stare, F. J.
  3. J. Boil. Chem. v.247 The effects of palmitic acid, oleic acids, on the property and compositions of the very low density lipoproteins secreted by the liver Heimberg, M.;Wilcox, H. G.
  4. J. Lipid Res. v.26 Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man Mattson, F. H.;Grundy, S. M.
  5. J. Lipid Res. v.31 Dietary influences on lipids and lipoproteins Grundy, S. M.;Denke, M. A.
  6. N. Engl. J. Med. v.323 Effect of dietary trans fatty acid on high-density and low-density liporotein cholesterol levels in healthy subjects Mensink, R. P.;Katan, M. B.
  7. Am. J. Clin. Nutr. v.56 Trans fatty acid intake in relation to serum lipid concentrations in adult men Troisi, R.;Willett, W. C.;Weiss, S. T.
  8. Am. J. C.I. in Nutr. v.59 Dietary trans fatty acids : effects on plasma lipids and lipoproteins of healthy men and women Judd, J. T.;Clevidence, B. A.;Muesing, R. A.;Wittes, J.
  9. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowery, O. H.;Rosebrough, N. J.;Farr, A. L.;Randall, R. J.
  10. Clin. Chem. v.19 Stable reagents for determination of serum triglycerides by a calorimetric Hantzsh condensation method Foster, L. B.;Dunn, R. T.
  11. J. Biol. Chem. v.234 Phosphorus assay in column chromatography Bartlett, G. R.
  12. J. Biol. Chem. v.226 A simple method for the isolation and purification of total lipids from animal tissues Folch, J.;Lees, M.;Stanley, G. H. S.
  13. Biochem. Med. v.20 Lipid composition and metabolic activity of the microsomal fractions from the arterial and liver tissues of swine Cho, B. H. S.;Kummerow, P. A.