Journal of Plant Biology
- 제39권1호
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- Pages.63-69
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- 1996
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- 1226-9239(pISSN)
2계단 배양방법을 이용한 인삼 모상근의 대량배양과 Ginsenoside 생산
Mass Culture and Ginsenoside Production of Ginseng Hairy Root by Two-Step Culture Process
- 고경민 (전남대학교 생물학과 및 호르몬연구센타) ;
- 양덕춘 (한국인삼연초연구원) ;
- 박지창 (한국인삼연초연구원) ;
- 최강주 (한국인삼연초연구원) ;
- 최광태 (한국인삼연초연구원) ;
- 황백 (전남대학교 생물학과 및 호르몬연구센타)
- Ko, Kyeong-Min (Department of Biology and Hormone Research Center, Chonnam National University) ;
- Yang, Deok-Chun (Korea Ginseng and Tobacco Research Institute) ;
- Park, Ji-Chang (Korea Ginseng and Tobacco Research Institute) ;
- Choi, Kang-Ju (Korea Ginseng and Tobacco Research Institute) ;
- Choi, Kwang-Tae (Korea Ginseng and Tobacco Research Institute) ;
- Hwang, Baik (Department of Biology and Hormone Research Center, Chonnam National University)
- 발행 : 1996.03.01
초록
인삼의 모상근 HRB-15 clone을 3L 용량의 생물반응기에서 배양하여 ginsenoside 생산량을 증진시킬 수 있는 방법을 찾고자 본 연구를 수행하였다. 모상근을 광배양하면 암배양에 비해 생장이 30% 정도 억제되지만 ginsenoside 함량(ginsenoside-Rb1, -Rb2, -Rc, -Rd, -Re, -Rg1)은 크게 증가되어 암배양시보다 2배 높은 1.10%로 나타났으며, 이때에는 ginsenoside-Re의 함량이 매우 높은 반면 다른 ginsenoside들의 함량은 암배양시 보다 다소 감소하는 경향이었다. 또한 초기 10일간 암배양한 후 20일간 광배양하는 방법을 기준으로 sodium acetate를 농도별로 처리하여 본 결과, sodium acetate의 농도가 증가할수록 모상근의 생장이 억제되고 ginsenoside 함량도 감소하였으며, 배양배지내 인산의 농도를 변화시켜 본 결과, 1.25 mM
A hairy root clone of Panax ginseng C.A. Meyer, HRB-15 was cultured iu various conditions with 3 L bubble type bioreactor to enhance both growth and ginsenoside production. The hairy roots were more rapidly grown under the dark condition than under the light condition. However, total amount of ginsenoside of hairy roots cultured under the light for 30 days increased 2 folds as compared with the dark condition and was 1.10% based on 6 ginsenosides. Especially, ginsenoside-Re was significantly increased and some ginsenosides except for ginsenoside-Re was slightly reduced. Also, the growth of hairy roots decreased about 30% as compared with the dark condition. In contrast, addition of sodium acetate led to decreased production of ginsenoside and growth of hairy roots under light condition. The influence of potassium dihydrogenphosphate concentration was examined in MS medium and a 1.25 mM concentration was found to be the most appropriate for growth and ginsenoside production under light condition. Two-step process of hairy roots culture with yeast elicitation or without ammonia in culture medium was developed to enhance growth and giusenoside synthesis.
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