Chitosan이 사과 겹무늬썩음병균 Botryosphaeria dothidea의 생육에 미치는 영향

  • 이승지 (경북대학교 자연과학대학 유전공학과) ;
  • 엄재열 (경북대학교 농과대학 농생물학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과)
  • Published : 1996.06.01

Abstract

To examine the potential utilization of chitosan, the biodegradable natural polymer, as a control agent of apple white rot caused by Botryosphaeria dothidea in a new control measure by coating it on the diseased branches, the various antifungal activities of chitosan was investigated. Chitosan showed significant inhibitory effect on the mycelial growth of B. dothidea, along with the morphological changes including hyphal swelling and ultrastructural changes on solid PDA medium. In liquid PD broth medium, the chitosan showed more significant effect on the growth of B. dothidea also forming cell clusters indicating affection on the hyphal extension. The growth of B. dothidea was inhibited more than 90% at the concentration of 1.0 mg/ml. Chitosan also detained the spore germination and induced the morphological change of germ tubes. Glucosamine, monomer of chitosan, did not affect on the growth of B. dothidea indicating the antifungal activity was caused by chitosan polymer.

Keywords

References

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