Biotechnology and Bioprocess Engineering:BBE
- Volume 1 Issue 1
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- Pages.51-53
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- 1996
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- 1226-8372(pISSN)
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- 1976-3816(eISSN)
High Cell Density Cultivation of Pseudomonas oleovorans for the Production of Poly(3-Hydroxyalkanoates)
- Lee, Sang-Yup (Department of Chemical Engineering and Bioprocess Engineering Research Center Korea Advanced Institute of Science and Technology)
- Published : 1996.12.01
Abstract
Fed-batch culture of Pseudomonas oleovorans was carried out for the production of medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using octanoate as a carbon source. Octanoate and the salt solution containing ammounium sulfate and magnesium sulfate were intermittently fed in the course of fermentation. Cell mass and PHA concentrations of 42.8 and 16.8g/L, respectively, could be obtained in 40 h. The PHA content and the PHA productivity were 39.2% and 0.42 g PHA/L-h, respectively. The yields of cell mass and PHA were 0.71 g dry cell mass/g octanoate and 0.28g PHA/g octanoate, respectively. Therefore, octanoate can be used for the production of MCL-PHAs to a high concentration with high productivity.
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