Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Effect of Ginseng Saponin on Gap Junction Channel Reconstituted with Connexin 32

  • Hong, Eun-Jung (Department of Biochemistry, Yeungnam University) ;
  • Huh, Keun (Department of Pharmacology, Yeungnam University) ;
  • Rhee, Seung-Keun (Department of Biochemistry, Yeungnam University)
  • Published : 1996.08.01
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Abstract

Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an established in vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1 % (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.

Keywords

References

  1. J. Membr. Biol. v.116 Connexin family of gap junction proteins Beyer,E.C.;Paul,D.L.;Goodenough,D.A.
  2. Dev. Biol. v.68 Modulation of cell junctions during differentiation of the chicken otocyst sensory epithelium Ginzberg,R.D.;Gilula,N.B.
  3. J. Cell Biol. v.107 Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions Goodenough,D.A.;Paul,D.L.;Jesaitis,L.
  4. J. Membr. Biol. v.109 Transport-specific isolation of large channels reconstituted into lipid vesicles Harris,A.L.;Walter,A.;Zimmerberg,J.
  5. Gap Junctions Hertzberg,E.L.;Johnson,R.G.
  6. Korean J. Ginseng Sci. v.17 no.3 Several physiological functions and metabolism of ginsenosides in the animal body Joo,C.N.
  7. Nature v.227 Cleavage of structural proteins during the assembly of the gead of bacteriohage T4 Laemmli,U.K.
  8. Physiol. Rev. v.61 Junctional intercellular communication: The cell-to-cell membrane channel Loewenstein,W.R.
  9. Biochim. Biophys. Acta. v.560 Junctional intercellular communication and the control of growth Loewenstein,W.R.
  10. Japanese J. Cancer & Chemotherapy v.21 no.15 Relationship between antiproliperative activity for target acetylenic alcohol, panaxydol, and its affinity for target cell membrane Matsunaga,H.;Saita,T.;Nagumo,F.;Mori,M;Katano,M.
  11. Biosci. Biotech. Biochem. v.58 no.9 Nerve growth factor-like immunoreactive substance in Panax ginseng extract Murase,K.;Yamamoto,T.;Hayashi,K.
  12. J. Biol. Chem. v.269 no.9 Cyclic AMP-dependent phosphorylation of an immunoaffinity-purified homotetrameric IP3 receptor increases Ca$^{2+}$ influx in reconstituted lipid vesicles Nakade,S.;Rhee,S.K.;Hamanaka,H.;Mikoshiba,K.
  13. J. Biol. Chem. v.259 no.3 Vasopressin-angiotensin II- and α₁-adrenergic-induced inhibition of Ca$^{2+}$ transport by rat liver plasma membrane vesicles Prpic,V.;Green,K.C.;Blackmore,P.F.;Exton,J.H.
  14. FASEB J. v.3 Purification of connexin32 by monoclonal antibody immunoaffinity chromatography Rhee,S.K.;Paul,D.;Harris,A.L.
  15. Biol. Pharm. Bull. v.17 no.5 Inhibition of tumor angiogenesis and mutagenesis by a saponin of Panax ginseng, ginsenoside-Rb2 Sato,K.;Mochizuki,M.;Saiki,I.;Yoo,Y.C.;Samukawa,K.;Azuma,I.
  16. The cell surface in animal embryogenesis Cell coupling and cell communication during embryogenesis Sheridan,J.D.;Poste,H.(ed.);Nicholson,G.L.(ed.)
  17. Annu. Rev. Physiol. v.47 Physiology and pharmacology of gap junctions Spray,D.C.;Bennett,M.V.L.
  18. Chinese drugs of plant origin: chemistry, pharmaclogy and use in traditional and modern medicine Tang,W.C.;Eisenbrand,G.
  19. Cancer Invest. v.6 Mechanisms of tumor promotion: potential role of intercellular communication Trosko,J.E.;Chang,C.C.;Metcalf,A.
  20. Cancer Res. v.48 Further evidence for the involvement of gap junctional intercellular communication and maintenance of transformed foci in BALB/c 3T3 cells Yamasaki,H.;Katoh,F.
  21. Nippon Jizo Gakkai Shi. v.36 no.1 Inhibitory effect of ginseng on proliferation of cultured mouse mesangial cells Yokozawa,T.;Iwano,M.;Dohi,K.;Hattori,M.;Oura,H.
  22. Biomed. Res. v.11 Ginseng rootevidence for numerous regulatory peptides and insulinotropic activity Zhang,T.;Hoshino,K.;Iguchi,K.;Ishikawa,J.;Mochizuki,T.;Takatsuka,N.;Yanaihara,C.;Yokota,M.;Greeley,G.H.;Yanaihara,N.