Archives of Pharmacal Research
- 제19권1호
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- Pages.12-17
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- 1996
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Important Role of Glutathione in Protecting Against Menadione-Induced Cytotoxicity in Rat Platelets
- Cho, Youn-Sook (College of Pharmacy, Seoul National University) ;
- Seung, Sang-Ae (College of Pharmacy, Seoul National University) ;
- Kim, Mee-Jeong (College of Pharmacy, Seoul National University) ;
- Lee, Joo-Young (College of Pharmacy, Seoul National University) ;
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Chung, Jin-Ho-Chung
(College of Pharmacy, Seoul National University)
- 발행 : 1996.02.01
초록
Our previous studies demonstrate that menadione (MEN) is cytotoxic to platelets of rats by depleting glutathione (GSH). In order to clarify whether GSH has a role in protecting against menadione-induced cytotoxicity, the effect of GSH depletors as well as GSH precusors on menadione-induced cytotoxicity was investigated. Cysteine and dithiothreitol (DTT) prevent MEN-induced cytotoxicity in a dose-dependent manner, as determined by LDH leakage and change in turbidity. When platelets were treated with 1-chloro-2,4-dinitrobenzene (CDNB) and diethylmaleate (DEM), both of which deplete intracellular GSH, MEN-induced cytotoxicity was potentiated in the CDNB-treated paltelets, but not in the DEM-treated platelets. These data suggest that the GSH in platelets plays an important role in protecting against cytotoxicity induced by menadione.