Mass Production of Resting Egg of Korean Rotifer, Brachionus plicatilis (L and S-type)

한국산 Rotifer, Brachionus plicatilis (L과 S-type)의 내구란 대량생산

Mass production of resting egg of the rotifer, Brachionus plicatilis was carried out in 1 $m^3$ tank for L-type rotifer and in 1 $m^3$ and 4 $m^3$ tank for S-type rotifer. L-type rotifer was fed on concentrated Chlorella + baker's yeast and only baker's yeast for 15 days. S-type rotifer was fed on concentrated Chlorella in 1 $m^3$ tank for 7 days and frozen Chlorella + baker's yeast in 4 $m^3$ for $6\~8$ days. Total number of resting egg and number of resting egg from $10^8$ rotifers and 1 g dry weight diet for L-type rotifer fed on Chlorella + baker's yeast were $54.5{\times}10^6$ eggs, $30.5{\times}10^6$ eggs and $100{\times}10^3$ eggs, respectively. These were higher than those of rotifer fed on baker's yeast only. Total number of resting egg and number of resting egg from $10^8$ rotifers and 1 g dry weight diet for S-type rotifer fed on concentrated Chlorella in 1 $m^3$ tank were $50\~104{\times}10^6$ eggs, $47\~82{\times}10^6$ eggs and $136\~260{\times}10^3$ eggs, respectively. In 4 $m^3$ tank with frozen Chlorella + baker's yeast, these were $149\~567{\times}10^6$ eggs, $36\~123{\times}10^6$ eggs and $131\~338{\times}10^3$ eggs, respectively. This result suggests that S-type rotifer are better than L-type rotifer for the mass production of resting egg of the Korean rotifer.

한국산 B. plicatilis, L-type rotifer와 S-type rotifer의 내구란 대량생산은 L-type rotifer의 경우 15일 동안 농축 Chlorella + 빵효모 혼합구와 빵효모 단독구로 하였고 S-type rotifer는 7일동안 1 $m^3$ 수조에서 농축 Chlorella로, $6{\~}8$일 동안 4 $m^3$ 수조에서 냉동 농축 Chlorella + 빵효모로 대량생산하였다. L-type rotifer 내구란 대량생산 실험에서 농축 Chlorella + 빵효모 혼합구가 총 내구란 생산 $54.5{\times}10^6$개, $10^{8}$ rotifers 당 내구란 생산 $30.5{\times}10^6$개, 먹이 건조 중량 1g 당 내구란 생산 $100{\times}10^3$개로 빵효모 단독구 보다 높게 나타났다. S-type rotifer의 내구란 대량생산은 4 $m^3$ 수조에서 냉동 농축 Chlorella + 빵효모를 먹이로 총 내구란 생산 $149{\~}567{\times}10^6$개, $10^{8}$ rotifers당 내구란 생산 $36{\~}123{\times}10^6$개, 먹이 건조 중량 1 g당 내구란 생산 $131{\~}338{\times}10^3$개를 생산하여 1 $m^3$ 수조에서 농축 Chlorella를 단독 먹이로 생산한 경우 보다 높았다.