Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Percutaneous Absorption of Recombinant h-EGF through Normal, Stripped and First-Degree Burn Skin

상피세포 성장인자의 경피흡수: 정상피부, 각질제거피부 및 화상피부에 있어서

  • Cho, Ae-Ri (College of Pharmacy, Duksung Women's National University) ;
  • Lee, Jung-Uk (College of Pharmacy, Seoul National University) ;
  • Ahn, Byung-Lak (College of Pharmacy, Seoul National University) ;
  • Chung, Joo-Young (R & D Center, Dae Woong Pharmaceutical Co., Ltd.) ;
  • Koh, Yeo-Wook (R & D Center, Dae Woong Pharmaceutical Co., Ltd.) ;
  • Shim, Chang-Koo (College of Pharmacy, Seoul National University)
  • 조애리 (덕성여자대학교, 교양학부) ;
  • 이정욱 (서울대학교 약학대학 약제학교실) ;
  • 안병락 (서울대학교 약학대학 약제학교실) ;
  • 정주영 (대웅제약 중앙연구소) ;
  • 고여욱 (대웅제약 중앙연구소) ;
  • 심창구 (서울대학교 약학대학 약제학교실)
  • Published : 1996.03.20
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Abstract

In vivo and in vitro skin permeation of $recombinant^{125}$ I-EGF through normal, stripped and the first degree burn skin were studied. The in vitro skin permeation rate through the first degree burn skin $(296\;cpm/cm^2/hr)$ and the stripped skin $(1131\;cpm/cm^2/hr)$ were 3.5 times and 13 times higher, respectively, as compared with the one through normal skin. In vivo absorption study with the first degree burn skin, the peak concentration of EGF in the skin was achieved at 1-3 hr and decreased afterward up to 8 hr with an elimination constant of $1.31{\times}10^{-3}\;g/ml/hr$. To investigate the higher elimination rate of EGF in burn skin, binding and metabolism studies were conducted. No significant metabolism of EGF in burn skin $(100^{\circ}C,\;5-second\;burning)$ was observed. With the presence or unlabelled-EGF $^{125}I-EGF$ permeation through the burn skin showed higher permeation rate than the one without unlabelled-EGF. The result nay indicate that EGF-receptor binding play a role in determining the skin permeation rate.

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