생약학회지 (Korean Journal of Pharmacognosy)
- 제27권4호
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- Pages.301-308
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- 1996
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
갈퀴꼭두선이의 Hairy Root 배양에 의한 Anthraquinone계 색소생산 연구(II)
Production of Anthraquinone Derivatives by Hairy Roots of Rubia cordifolia var. pratensis
- Kim, You-Sun (College of Pharmacy, Duksung Women's University) ;
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Shin, Seung-Won
(College of Pharmacy, Duksung Women's University)
- 발행 : 1996.12.30
초록
Hairy roots induced from stems of Rubia cordifolia var. pratensis were cultured in the liquid medium under a variety of auxins to find the optimal condition for the growth and production of pigments. Culture of the hairy roots on NN liquid medium containing NAA 0.5 mg/l was best for growth of hairy roots. Production of yellow anthraquinone derivatives and purpurin in hairy roots was enhanced by the culture on NN liquid medium without auxins. Effects of L-phenylalanine, L-tyrosine and juglone, synthesized via the shikimic acid pathway, on growth and production of pigments in hairy roots were studied in the present study. Concentration of exogeneous L-phenylalanine. L-tyrosine and juglone in liquid culture system of hairy root containing NAA 0.1 mg/l was decreased quickly in its early stages of the culture period. Addition of juglone to NN liquid medium containing NAA 0.1 mg/l enhanced the productivity of pigments in hairy roots.