Studies on Antitumor Effect and Synergistic Action of Natural Products with Anticancer drugs against Hepatic Tumors

생약의 간암세포에 대한 항종양효과와 항암제와의 상승작용

The antitumor effect of 柴胡(Bupleuri Radix : BP), 茵陳(Artemisiae capillaris Herba; ACH) 및 蒲公英(Taraxaci Herba; TH) and 蒲公英 EE層(Ethyl ether layer of TH; EETH) on human hepatocytes such as Hep G2, PLC and Hep 3B, and synergistic action with the anticancer drugs, that is, mitomycin(MMC), cisplatin(CPT) and 5-fluorouracil(5-FU) were studied by the method of MTT. The results were obtained as follows: 1. $IC_{50}$ against Hep G2, PLC and Hep 3B was $15.5{\mu}g/ml$, $25.4{\mu}g/ml$ and 31.25 in MMC, $92.5{\mu}g/ml$, $50.2{\mu}g/ml$ and $62.5{\mu}g/ml$ in CPT and $125{\mu}g/ml$ in 5-FU respectively. 2. Cytotoxic effect on Hep G2 was obvious in BP-treated group, synergistic action was most effective in TH-treated group or with MMC. 3. Cytotoxic effect on Hep 3B was obvious in ACH-treated group, synergistic action was most effective in ACH-treated group or with MMC. 4. Cytotoxic effect on PLC was obvious in ACH-treated group, synergistic action was most effective in TH-treated group or with MMC. From above results it was concluded that ACH showed the best antitumor effect against PLC and Hep 3B, BP aganst Hep G2 and also synergistic effect was most effective with MMC, which indicates that it is necessary to seperate the antitumor substances in ACH.

연구배경: 간암은 우리나라에서 암의 사망율 중 2위인데 한방임상에서 간암의 치료에 인진, 포공영, 시호등이 자주 사용되고 있는데 이들 한약의 간암세포에 대한 항종양효과와 간암치료에 사용되는 항암제와의 상승작용을 실험적으로 입증할 필요가 있어 실험에 착수하였다. 방법: in vitro에서 시호, 인진, 포공영 및 포공영 EE층의 인체 간암세포인 PLC(hepatoma), Hep 3B(hepatocellular carcinoma) 및 Hep G2(hepatocellular carcinoma) 등에 대한 세포독성과 간암치료에 다용되는 mitomycin C(MMC), cisplatin(CPT), 5-fluorouracil(5-FU) 등의 항암제와의 상승효과를 MTT법에 의해 측정하였다. 결과: 인진은 비교적 PLC와 Hep 3B에 대하여 항종양효과가 있고, 시호는 Hep G2에 대해 보다 효과적이고, 항암제중 MMC와의 상승적 작용이 뚜렷하여 앞으로 인진으로 부터 항암활성물질의 분리가 필요하며 한약과 항암제와의 병용투여 가능성을 제시한다고 사료된다.