Journal of Yeungnam Medical Science
- Volume 12 Issue 1
- /
- Pages.124-134
- /
- 1995
- /
- 2799-8010(eISSN)
The Effect of EDTA and Fetal Cord Serum Supplementation to Ham's F-10 Culture Medium on Developmental Potential of Mouse Embryos In Vitro
Ham's F-10 배양액에 첨가된 태아제대혈청과 EDTA가 백서 수정란의 분할에 미치는 영향
- Kim, Byeong-Seog (Department of Obstetrics and Gynecology College of Medicine, Yeungnam University) ;
- Lee, Young-Gi (Department of Obstetrics and Gynecology College of Medicine, Yeungnam University) ;
- Park, Yoon-Kee (Department of Obstetrics and Gynecology College of Medicine, Yeungnam University) ;
- Lee, Tae-Hyung (Department of Obstetrics and Gynecology College of Medicine, Yeungnam University) ;
- Lee, Sung-Ho (Department of Obstetrics and Gynecology College of Medicine, Yeungnam University)
- 김병석 (영남대학교 의과대학 산부인과학교실) ;
- 이영기 (영남대학교 의과대학 산부인과학교실) ;
- 박윤기 (영남대학교 의과대학 산부인과학교실) ;
- 이태형 (영남대학교 의과대학 산부인과학교실) ;
- 이승호 (영남대학교 의과대학 산부인과학교실)
- Published : 1995.06.30
Abstract
It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown that the addition of biologic fluids, such as blood serum, of various origins, improved fertilization and early cleavage rates in numerous species. The purpose of this study is to attempt to measure developmental potential of mouse eggs fertilized and cleaved in Ham's F10 culture medium containing a chelating agent, EDTA and fetal cord serum. In this study, we selected 40 female mice and 20 male mice, and investigated optimal serum concentrations for mouse embryo growth. Two cell stage mouse embryos were cultured in Ham's F-10 medium, Ham's F-10 medium with various concentrations of EDTA, or Ham's F-10 medium with EDTA and 10% human cord serum. Developmental ratios to morula in Ham's F-10 medium containing various concentrations of EDTA and/or 10% fetal cord serum were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to blastocyst in Ham's F-10 containing 10% fetal cord serum and
Ham's F-10 기본 배양액과 이 배양액에 여러가지 농도의 EDTA와 태아제대혈청을 단독 또는 같이 혼합한 배양액을 얻어 각각의 배양액에서 5-10주된 백서를 이용하여 2 세포기 배아의 단계적 분할정도를 96시간 동안 관찰하였다. Ham's F-10 기본 배양액에 비해 태아제대혈청이나 EDTA