Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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des-$Asp^4$-Amastatin, MRK-22 as an Inhibitor of Aminopeptidase M produced by Streptomyces sp. SL20209

  • Kho, Yung-Hee (Microbial Chemistry RG. and Protein Engineering R.G., Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Ko, Hack-Ryong (Microbial Chemistry RG. and Protein Engineering R.G., Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Chun, Hyo-Kon (Microbial Chemistry RG. and Protein Engineering R.G., Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Seung-Ho (Protein Engineering R.G., Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Sung, Nack-Kie (Department of Food Science and Technology, Gyeongsang National University)
  • Published : 1995.06.01
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Abstract

MRK-22, an inhibitor of aminopeptidase M was isolated from the culture broth of Streptomyces sp. SL20209. The structure of MRK-22 was defined to be 3-amino-2-hydroxy-5-methylhexanoyl-valyl-valine, des-$Asp^4$-amastatin, by spectroscopic analysis and this was also confirmed by solid phase synthesis of the inhibitor. The molecular formula and weight of MRK-22 were $C_17H_33N_3O_5$ and MW 359($M^+$), respectively, and its $IC_50$ value against hog kidney AP-M was 0.79 $\mu$ g/ml.

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