Journal of Microbiology and Biotechnology
- Volume 5 Issue 2
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- Pages.87-91
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Molecular Cloning of Acinetobacter sp. WC-17 Gene Encoding Chitinase
- SOON-DUCK HONG (Department of Microbiology, College of Natural Science, Kyung-Pook National University) ;
- SHIN, WOO-CHANG (Department of Microbiology, College of Natural Science, Kyung-Pook National University) ;
- DONG-SUN LEE (Department of Microbiology, College of Natural Science, Kyung-Pook National University) ;
- JONG-GUK KIM (Department of Microbiology, College of Natural Science, Kyung-Pook National University)
- Published : 1995.04.01
Abstract
The chitinase gene was cloned from Acinetobacter sp. WC-17 for investigating the genetic control and enzymatic properties of bacterial chitinase. A genomic library of Acinetobacter sp. WC-17 was prepared in E.coli JM109 by using pUC18 as a vector. The chitinase-positive clone containing 3.2kb insert fragment was obtained from 5, 000 insert-bearing transformants. The optimum pH and temperature of cloned enzyme were 6.0 and