Abstract
The effects of plant hormones (NAA, $GA_3$ and BA) and light qualities (white, red, green and blue light) on the changes of reducing sugar contents and invertase isozyme activities in leaves of maize (Zea mars L.) and mung bean (Phseolw radiatus L.) seedlings were investigated. NAA accelerated the increase of reducing sugar contents and invertase isozyme activities, on the contrary, $GA_3$ had little effect in the accumulation of reducing sugar and in the increase of enzyme activities from the leaves of maize and mung bean seedlings. On the other hand, BA accelerated an increase in the activities of the invertase isozyme from the leaves of mung bean seedlings whereas it had little effect in the increase of the enzyme activities from those of maize seedlings. The accumulation of reducing sugar in leaves of both seedlings was promoted by red light irradiation compared to white light irradiation, while the activities of the enzyme were little affected by various light Qualities. In the simultaneous applications of plant hormone and light quality, NAA with white light was very effective in the increase of reducing sugar contents and the enzyme activities from the leaves of mung bean seedlings, whereas NAA application with blue light showed a prominent enhancement in the reducing sugar contents and the enzyme activities from those of maize seedlings. These results suggest that plant hormone, particularly NAA, may be a more important factor than various light Qualities in the stimulation of invertase activity.
여러 파장의 광선(백색, 적색, 녹색 그리고 청색광)과 NAA, $GA_3$, BA 등의 식물호르몬을 옥수수와 녹두 유식물의 잎에 처리하여 환원당 축적과 invertase isozymes 활성에 미치는 효과를 조사하였다. NAA는 옥수수와 녹두 잎의 환원당과 invertase isozymes 활성의 증가를 촉진하였으나, $GA_3$는 환원당 축적과 효소의 활성증가에 효과적이지 못하였다. 한편 BA는 녹두 잎의 imvertase isozmes의 활성증가를 유도하는 반면, 옥수수 잎에 있어서는 큰 영향을 미치니 못하였다. 환원당의 축적에 있어서 적색광은 백색광에 비하여 효과적이였으나, invertase isozymes의 활성증가에 있어서는 여러 파장의 광선 모두가 촉진효과를 보여주지 않았다. 식물호르몬과 광선의 동시처리에 있어서 NAA와 백색광 동시처리는 녹두 잎의 환원당 축척 및 invertase isozymes의 활성증가에 매우 효과적이였으며 NAA와 청색광 동시처리는 옥수수 잎의 환원당 축척과 invertase isozymes의 활성증가에 매우 효과적이었다. 이상의 결과로 보아 옥수수와 녹두 잎의 환원다 축척 및 inverses isozymes의 활성증가에 있엇 식물호르몬, 큭히 NAA는 여러 파장의 광선보다 더 중요한 요인인 것으로 사료된다.