Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Flavonoid Analysis from the Leaves of Eucommia ulmoides

두충나무잎의 생리활성 Flavonoid 분석

  • Published : 1995.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Three flavonoid compounds, astragalin(1), isoquercitrin(2) and quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside(3)$ isolated from the leaves of Eucommia ulmoides were identified and quantified by HPLC. All flavonoids were well separated on a ${\mu}-Bondapak\;C_{18}$ column with a mobile phase composed of THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658). The contents of compound 1 in the methanol extract and n-butanol fraction were 0.09%(w/w) and 0.46%(w/w), of compound 2 were 0.08%(w/w) and 0.48%(w/w), and of compound 3 were 0.40%(w/w) and 1.22%(w/w), respectively.

기호음료 및 약용으로 사용되는 두충나무잎의 품질 평가를 위한 HPLC로서 함유된 flavonoid 화합물을 정성, 정량 분석하였다. 즉 두충나무잎의 n-BuOH 분획으로 부터 astragalin, isoquercitrin, quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside$의 3종 flavonoid 성분들을 분리하였으며, 이들 화합물은 LPLC의 THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658) 혼합용매에서 양호한 분리능을 나타내었다. MeOH 엑스와 n-BuOH 분획 중에 astragaline의 함량은 각각 0.09%(w/w), 0.46%(w/w), isoquercitrin은 0.08%(w/w), 0.48(w/w), quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside$은 0.40%(w/w), 1.22%(w/w) 함유되어있다.

Keywords

References

  1. 대한식물도감 이창복
  2. 중약대사전 小學棺
  3. 원색화한약도감(하) 雜波恒雄
  4. 원색천연약물대사전 (상) . 김재길
  5. 생약학회지 v.6 혈압강하제 국산자원생약의 개발 연구 정명현;박정완
  6. 생약학회지 v.18 두충나무의 항당뇨활성에 관한 연구 홍남두;노영수;원도희;김남재;조보선
  7. 생약학회지 v.19 두충나무의 일반 약리활성 연구 홍남두;노영수;김종우;원도희;김남재;조보선
  8. J. Med. Pharm. Soc. for Wakan-Yaku v.3 Studies on Tu-Chung leaves (I) Namba,T.;Hattori,M.;Yie,J.;Ma,Y.;Nomura,Y.;Katamura,K.;Lu,W.
  9. J. Med. Pharm. Soc. for Wakan-Yaku v.4 Studies on Tu-Chung leaves (II) Ma,Y.;Yie,J.;Hattori,M.;Kaneko,S.;Nomura,Y.;Wakaki,K.;Namba,T.
  10. Gazzetta Chimica Italiana v.108 Iridoids. XXVI. Ulmoside, a new iridoid from Eucommia ulmloides Bianco,A.;Bonini,C.;Guiso,M.;lavarone,C.;Trogolo,C.
  11. Tetrahedron v.30 Structure of eucommiol, a new cyclopentenoid-tetrol from Eucommia ulmoides Bianco,A.;lavarone,C.;Trogolo,C.
  12. Chem. Pharm. Bull. v.31 The constituents of Eucommia ulmoides I. Isolation of (+)-medioresinol di-O-$\beta$-D-glucopyranoside Deyama,T.
  13. J. Am. Chem. Soc. v.98 Isolation and synthesis of pinoresinol diglucoside, a major anti-hypertesive principle of Tu-Chung (Eucommia ulmoides) Charles,J.S.;Ravikumar,P.R.;Huang,F.C.
  14. Chem. Pharm. Bull. v.33 The constituents of Eucommia ulmoides II. Isolation and structures of three new lignan glycosides Deyama,T.;Ikawa,T.;Nishibe,S.
  15. Chem. Pharm. Bull. v.34 The constituents of Eucommia ulmoides III. Isolation and structure of a new lignan glycosides Deyama,T.;Ikawa,T.;Kitagawa,S.;Nishibe,S.
  16. Tetrahedron Lett. v.50 Ulmoprenol, a new type C₃ polyprenoid from Eucommia ulmoides Horii,Z.;Ozaki,Y.;Nagao,K.;Kim,S.
  17. Arch. Pharm. Res. v.14 Phenolic compounds of the leaves of Eucommia ulmoides Young,H.S.;Park,J.C.;Park,H.J.;Lee,J.H.;Choi,J.S.
  18. Z. Allg. Microbiol. v.16 Testing of antiviral compounds against Mengo virus infection of Mice. A 2step procedure of in vivo screening Veckenstedt,A.;Horn,M.
  19. Progress in drug research v.22 Antiviral agent Swallow,D.L.;Jucker,E.(ed.)
  20. Yakhak Hoeji v.34 Pharmacological activities of flavonoids (II)-Relationships of anti-inflammatory and antigranulomatous actions Kim,C.J.;Su,S.K.;Joo,J.H.;Cho,S.K.
  21. 생물활성천연물질 柴田承二
  22. J. Korean Son. Food Nutr. v.21 The pharmaco-chemical study on the plant of Ixeris spp. 2. Flavonoid and free amino acid composition of Ixeris sonchifolia Young,H.S.;Im,K.S.;Choi,J.S.
  23. J. Natural Products v.57 Synthesis of quercetin-3-O-$\beta$-D-glucopyranosyl-(1-2)-$\beta$-D-xylopuranoside via orthoester methodology Demetzos,C.;Skaltsounis,A.L.;Razanarnahefa,B.;Tillequin,F.