Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Methionine on Heart Lipid Peroxidation in Rat with Alcohol Administration

Ethanol 급여 흰쥐의 심장 지질과산화에 미치는 Methionine의 영향

  • 조수열 (영남대학교 식품영양학과) ;
  • 박은미 (영남대학교 식품영양학과) ;
  • 김명주 (신일전문대학 식품영양과) ;
  • 이미경 (영남대학교 식품영양학과) ;
  • 성인숙 (영남대학교 식품영양학과)
  • Published : 1995.08.01
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Abstract

This study was designed to investigate the effects of methionine(Met) on the activities of heart lipid peroxidation related enzymes in ethanol administrated rats. Male Sprague-Dawley rats were fed on diets containing one of the three levels of Met(0%, 0.3%, 0.9% of kg diet) and ethanol(2.5g/kg of body weight) was administrated as 25v/v% ethanol to ethanol treated groups orally. The rats were sacrificed after 5 and 10 weeks of feeding. Xanthine oxidase(XO) and catalase activities increased with ethanol administration and those activities were higher n Met excessive and deficiency group than those of Met normal group at 5 and 10 weeks dieting. Superoxide dismutase(SOD) activity in heart decreased significantly in Met deficiency and Met excessive group as compared to that of control. Glutathione peroxidase(GSH-Px) activity in heart significantly decreased in Met deficiency group as compared to that of Met excessive and normal group. Glutathione S-transferase(GST) activity of heart tissue significantly increased by ethanol administration. Glutathione(GSH) content in heart decreased with ethanol administration and shwoed no significant differences with Met levels. Ethanol administration increased the content of lipid peroxide(LPO).

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References

  1. J. Nutr. v.115 Effect on susceptibility to ethanol toxicity in rat Zidenberg,C.;Hurley,S.;Lonnerdal,B.;Keen,C.
  2. Lab. Invest. v.52 Biology of disease : Effect of ethanol on the chemical and structural properties of biologic membranes Taraschi,T.F.;Rubin,E.
  3. Vitamin E A comprehensive treaties Molenaar,I.;Hulstaert,C.E.;Handonk,M.J.
  4. J. Nutr. v.115 Effect on Se deficiency on hydroperoxide-induced glutathione release from isolated perfused rat heart Xia,Y.;Hill,K.E.;Burk,R.F.
  5. J. Biol. Chem. v.257 Effect of de deficiency and vit. E deficiency on glutathione metabolism in isolated rat hepatocytes Hill,K.E.;Burk,R.F.
  6. J. Nutr. v.107 Protection against carbon tetrachloride-induced lipid ethane evolution Hafrman,D.G.;Hoekstra,W.G.
  7. J. Lab. Clin. Med. v.98 Ethanol induced lipid peroxidation : potentiation by chronic alcohol feeding and attenuation by methionine Shaw,S.;Jayatilleke,R.;ross,W.A.;Gordon,E.R.;Lieber,C.S.
  8. J. Nutr. v.107 Report of the American institute of nutrition : Ad Hoc committee on standards for nutritional studies American institute of nutrition
  9. J. Biol. Chem. v.193 Protein measurement with folin reagent Loery,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  10. J. Biol. Chem. v.224 The regulation of rat liver xanthine oxidase : Conversion in vitro of enzyme from dehydrogenase(type D) to oxidase (type C) Stripe,F.;Della,C.E.
  11. Eur. J. Biochem. v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund,S.;Marklund,G.
  12. Method of Enzymatic Analysis v.2 Catalase Aebi,H.;Ergmeyer,H.U.
  13. J. Lab. Clin. Med. v.70 Studies on the quantitative and quatitative charaterization of erythrocytes glutathione peroxidase Paglia,E.D.;Valentine,W.N.
  14. J. Biol. Chem. v.249 Glutathione S-transferase. The first enzymatic step in mercapturic formation Habig,W.H.;Pabst,M.J.;Jakoby,W.B.
  15. Anal. Biochem. v.93 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Ohkawa,H.;Ohishi,N.;Yaki,K.
  16. Arch. Biochem. Biophys. v.82 Tissue sulfhydryl group Ellman,G.L.
  17. Statistical methods(6th ed.) Snedecor,G.W.;Cochrane,W.G.
  18. Casarett and doull's toxicology(3rd ed.) Klaassen,C.D.;Amdur,M.O.;Doull,J.
  19. Gastroenterology v.98 Ethanol induced injury to the rat gastric mucosa Peter,R.;Beverleigh,T.;Jerone,D.;Robert,D.S.
  20. Am. J. clin. Nutr. v.41 Changes in hepatic superoxide dismutase activity in alcoholic monkeys Keen,C.L.;Tamura,T.;Lonnerdal,B.;Hurley,L.S.;Halsted,C.H.
  21. Toxicology v.45 Enhancement by glutathione depletion of ethanol-induced acute hepatotoxicity in viteo and in vivo Strubelt,O.;Younes,M.;Pentz,R.
  22. Eur. J. Biochem. v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund,S.;Marklund,G.
  23. J. Nutr. v.104 Effect of dietary Se and autoxidized lipid on the glutathione peroxide Se system of gastrointestina tract and other tissues in thr rat Reddy,K.;Tsappel,A.L.
  24. Am. J. Physiol. v.239 Physiological siginificance of GSH S-trandferases Kaplowitz,N.
  25. Alcol. Clin. Exp. Res. v.12 Depletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism Speisky,H.;Kera,Y.;Penttila,K.E.;Israel,Y.;Lindros,K.O.