Bacillus sp. SH-8과 Bacillus sp. SH-8M의 세포벽과 세포막에 미치는 pH의 영향

Effect of pH on the Cell Wall and Cell Membrane of Bacillus sp. SH-8 Bacillus sp. SH-8M

  • 심창환 (경민전문대학 식품영양과) ;
  • 정용준 (전주대학교 미생물학과) ;
  • 신원철 (강원대학교 발효공학과 및 생물산업소재연구센터)
  • 발행 : 1995.02.01

초록

Using the alkalophillic Bacillus sp. SH-8 and its mutant Bacillus sp. SH-8M capable of growing at the neutral pH, the amino acid compositions of the cell wall and cell membrane were studied at varying cultivation pH's. The pattem of protein electrophoresis was also tested. It was elucidated that the amino acids consisting of the cell wall were alanine, glutamic acid, lysine, aspartic acid, and meso-diaminopimelic acid. There was not any significant difference in the amino acid compositqon betweeo`two straqns regardless of the culture pH. As the results of HPLC ssay, glutamic acid and aspartic aciu accounted for more than 50% in the amqno acid composytqon of the cell wall. By the isolatqon of the crude cell membrane and the SDS-PAGE analysis, it was found that there was a considerable difference qn the protein pattern when the straqns were cultured at the neutral pH. In addition, by the two dimensional gel electrophoresis, it was confirmed that there was a difference in the protein patterns between two strains cultivated at the neutral pH medium but no difference at the alkaline medium.

키워드

참고문헌

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