아미노산과 대사산물들이 Serratia marcescens Biodegradative Threonine Dehydratase의 생합성에 미치는 영향

The Effects of Amino Acids and Metaolites on the Biosynthesis of Biodegradative Theronine Dehydratase in Serratia matcescens ATCC25419

  • 최병범 (연세대학교 이과대학 생화학과) ;
  • 김승수 (연세대학교 이과대학 생화학과 생물산업소재연구센터)
  • 발행 : 1995.02.01

초록

The effects of amino acids in growth media on the biosynthesis of Serratia marcescens biodegradative threonine dehydratase activity were examined. The enzyme activity was decreased by 44 and 34% by 10 mM isoleucine and valine, respectively, whereas it was increased approximately by 20% by 10 mM threonine. Among several metabolites tested, pyruvate increased the enzyme activity by 60% at 5 mM, but decreased the enzyme activity approximately by 20 to 70% above 20 mM. The enzyme activity was increased by 64% by 5 mM glyoxylate, whereas it decreased the enzyme activity approximately by 40 to 70% above 20 mM glyoxylate. The thiamine, monopyrrole derivative, also increased the enzyme activity by 84% at 50 $\mu $g/ml, but did not affected the enzyme activity above 300 $\mu $g/ml. cAMP increased the enzyme activity by 58% at 0.5 mM, but decreased the enzyme activity by 15% at 2 mM. These data suggested that the biosynthesis of Serratia marcescens biodegradative threonine dehydratase is regulated by concentrations of pyruvate, glyoxylate and cAMP.

키워드

참고문헌

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