Cathepsin B 저해물질을 생산하는 Streptomyces chromofuscus의 분리 및 동정

Isolation and Identification of Streptomyces chromofuscus Producing Cathepsin B Inhibitor

  • 발행 : 1995.10.01

초록

The aim of the present research program was to develop a strain of actinomycetes producing extracellular cathepsin B inhibitor. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying various physical and chemical pretreatments. An economical and effective method was developed for the screening of strains producing low molecular weight cathepsin B inhibitor, and consequently a strain (SMF28) among over 700 isolates was selected. Chemotaxonomic and numerical identification were carried out for the isolate. Fifty taxonomic unit characters were tested and the data were analyzed numerically using TAXON program. The isolate was identified as a strain of Streptomyces chromofuscus.

키워드

참고문헌

  1. J. Antobiot. v.22 Biologocal activities of leupeptins Aoyagi,T.;S.Miyata;M.Nanbo;F.Kojima;M.Matsuzaki;M.Ishizuka;T.Takeuchi;H.Umezawa
  2. J. Antibiot. v.25 Antipain, a new protease inhibitor isolated actinomycetes Suda,H.;T.Aoyagi;M.Hamada;T.Tekeuchi;H.Umezaswa
  3. J. Antibiot. v.23 Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes Umezawa,H.;T.Aoyagi;H.Morishma;S.Kunimoto;M.Matsuzaki;M.Hamada;T.Tekeuchi
  4. J. Antibiot. v.26 Elastainal, a new elastase inhibitor produced by actinmycetes Umezawa,H.;T.Aoyagi;A.Okura;H.Morishima;T.Takeuchi;Y.Okami
  5. Tetrahedron v.38 The structure of β-MAPI, a novel proteinase inhibitor Shin-Watanabe,T.;K.Fukuhara;S.Murao
  6. J. Antibot. v.23 Pepstatin, a new pepsin inhibitor produced by actinomycetes Umezawa,H.;T.Aoyagi;H.Morishma;M.Matsuzaki;M.Hamada;T.Tekeuchi
  7. J. Antibiot. v.26 A thermolysin inhibitor produced by actinomycetes. Phosphoramidon Suda,H.;T.Aoyagi;M.Hamada;H.Umezawa
  8. J. Antibiot. v.37 Histargin,a new carboxypeptidase B, produced by actinomycetes Umezawa,H.;T.Aoyagi;K.Ogawa;H.Iinuma;H.Naganaa;M.Hamada;T.Takeuchi
  9. Proteinases in Mammalian Cells and Tissues Cathepsin B and other thiol proteinases Barrett,A.J.(ed.)
  10. In Methods in Enzymology v.80 Cathepsin B, cathepsin H, and cathepsin L Barrett,A.J.;H.Kirschke
  11. Proteinases in mammalian cells and tissues Barrett,A.J.
  12. Science v.200 Muscular dystrophy: Inhibition of degeneration in vivo with protease inhibitors Stracher,A.;E.B.McGowan;S.A.Shafiq
  13. Arch. Biochem. Biophys. v.96 Increased lysosomal enzymes in genetic muscular dystrophy Tappel,A.L.;H.Zalkin;K.A.Caldwell;I.D.Desai;S.Shibko
  14. J. Biochem. v.98 Distribution of cathepsin B and H in rat tissues and peripheral blood cells Kominami,E.;T.Tsukahara;Y.Bando;N.Katunuma
  15. Int. J. Syst. Bacteriol. v.16 Methods for the characterzation of Streptomyces species Shirling,E.B.;D.Gottlieb
  16. J. Gen. Microbiol. v.129 A probability matrix for the identification of some streptomycetes Williams,S.T.;M.Goodfellow;E.M.H.Wellington;J.C.Vickers;G.Alderson;P.H.A.Sneath;M.Sackin;A.M.Mortimer
  17. J. Gen. Microbiol. v.129 Numerical classification of Streptomyces and related genera Williams,S.T.;M.Goodfellow;G.Alderson;E.M.H.Wellington;P.H.A.Sneath;M.Sackin
  18. Microbiosis Letters v.6 Preservation of actinomycete inoculum in frozen glycerol Wellington,E.M.H.;S.T.Williams
  19. J. Gen. Appl. Microbiol. v.16 Principle amino acids in the cell wall and their taxonomic significance Yamada,K.;K.Kamagata
  20. Appl. Microbiol. v.28 Simplifide approach to identification of aerobic actinomycetes by thin-layer chromatograph Staneck,J.L.;G.D.Robert
  21. J. MIcrobiol. Biotechnol. v.2 Numerical identification of a Streptomyces strain producing thiol protease inhibitor Kim,I.S.;H.T.Kim;A.C.Ward;M.Goodfellow;Y.C.Hah;K.J.Lee
  22. Kor. J. Microbiol. v.30 Numerical identification of a Streptomyces strain producing spores in submerged culture Rho,Y.T.;H.T.Kim;K.H.Oh;H.I.Kang;A.C.Ward;M.Goodfellow;Y.C.Hah;K.J.Lee
  23. Anal. Biochem. v.47 A new assay for cathepsin B1 and other thiol proteinases Barrett,A.J.
  24. Actinomycete taxonomy, special publication 6, Society for Industrial microbiology The chemotaxonomy of actinomycetes Lechevalier,H.A.;M.P.Lechevalier;A,Dietz(ed.);D.W.Thayer(ed.)
  25. Bergy's Manual of Systematic Bacteriology v.4 Streptomycetes and related genera Romaano,L.;S.T.Williams(ed.);M.E.Sharp(ed.);J.G.Holt(ed.)
  26. Kor. J. Appl. Microbiol. Biotechnol. Production and characterization of cathepsin B inhibitor from Streptomyces chromofuscus SMF28 Lee,H.S.;I.S.Kim;H.T.Kim;S.J.Yoon;K.J.Lee