한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권5호
  • /
  • Pages.559-564
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Cephalosporin C Acylase 생산균주의 분리 및 특성

Isolation and Charaterization of Microorganism Producing Cephalosporin C Acylase

  • 박용춘 (충북대학교 자연과학대학 미생물학과) ;
  • 김욱현 (충북대학교 자연과학대학 미생물학과) ;
  • 임재윤 (충북대학교 자연과학대학 미생물학과) ;
  • 김영창 (충북대학교 자연과학대학 미생물학과)
  • 발행 : 1995.10.01
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초록

Twenty microbial strains producing the acylase were isolated from soil by using Micrococcus luteus ATCC 9341 as an indicator strain, using either D-($\alpha $)-phenylglycine methylester and 7-aminocephalosporanic acid (7-ACA) or glutaric acid dimethylester and 7-ACA as substrates. Among the isolates, only one strain was turned out to be the 7-ACA producer from either cephalosporin C or glutaryl 7-ACA as the substrates by using the overlay of 7-ACA sensitive strain (SS5). 7-ACA produced from cephalosporin C by an isolate (APS20) was detected by high performance liquid chromatography. The isolated strain (APS20) was identified to Bacillus macerans on the basis of cellular fatty acid profile by gas chromatography. Bacillus macerans APS20 had no $\beta $-lacta-mase activity on cephalosporin C, and that is very important for the enzymatic production process of 7-ACA. However, this strain was resistant up to 100 $\mu $g/ml of cephalosporin C.

키워드

참고문헌

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