Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Toxic Effect of Cadmium on the Amphibian Axis Formation

카드뮴이 양서류의 체축 형성에 미치는 독성 효과

  • 김윤경 (서울대학교 사범대학 생물교육학과) ;
  • 정해문 (서울대학교 사범대학 생물교육학과)
  • Published : 1995.06.01
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Abstract

Effect of cadmium on the early amphibian development was analyzed through FETAX (Frog Embryo Teratogenesis Assay: Xenopus). Embryos manifested concentration-dependent mortality and realformations; shortage of anterior-posterior axis gut realformation, ocular anomalies, bent notochord, misshapen dorsal fin, and derreal blisters. The treatment with 1.5ppm cadmium solution caused 100% mortality and concentration of lppm did not kill the embryos that caused 100% anomaly. The teratogenic index (TI = LC50 /EC50) was 2.8 indicating that $CdCl_2$ is teratogenic for Xenopus laevis. Embryos that were pulsetreated with at early to late blastula stage (St. 3-9) and mid to late blastula stage (St. 6-10) showed relatively strong resistance to cadmium, but the embryos treated at gastrula stage (St. 10-13) showed high mortality. And the embryos treated at tailbud stage (after St. 25) showed highest mortality of any other early stages. Effects of temperature were studied through pulse- treatment during gastrula stage at $20^{\circ}C$ and $30^{\circ}C$. The embryos treated with 7.5ppm at $30^{\circ}C$ and 15ppm at $20^{\circ}C$ caused 100% mortality respectively, indicating that higher temperature had more severe toxic effect. One of the most peculiar effect of cadmium at gastrulation was distortion of the tail. The probable cause of toxic effect of Cd was discussed.

Keywords

References

  1. Comp. Biochem. Physiol. v.76C no.2 A comparison of the accumulaion and protein binding of environmental cadmium in the gills, kidney and liver of rainbow trout(salmo gairdneri Richardson) Dafydd,G.T.;Cryer,A.;de L.G.Solbe,J.F.;Kay,J.
  2. Laboratory Diagnosis of Disorders of the Fetus, Newborn, and Early childhood The FETAX test for teratogenicity of chemicals and environmental samples Hopfer,S.M.;Plowman,M.C.;Sunderman,F.W.Jr.;Sunderman,F.W.(ed.)
  3. Japanese J. of Hygine Contents of cadimium and zinc in organs of Itai-Itai disease patients and residents of Hokuriku District Ishizaki,A.;M.Fukushima;M.Sakamoto
  4. Toxicol. Appl. Pharmacol v.31 Effect of multiple doses of cadmium on glucose metabolism and insulin secretion in the rat Ithakissios,D.S.;Ghafgazi,T.;Menner,J.H.;Kessler,W.V.
  5. Japanese Journal of Physiology v.30 Effect of Cadmium on Active Sodium Transport by the Abdominal Skin and the Isolated Epidermis of the Bullfrog: Differences in Effects between Epidermal and Dermal Cadmium Application Makoto,T.;H.Hayashi
  6. Toxicol. and Appl. Pharmacol. v.74 Altered immune response during cadmium administration in mice Malave,I.;D.T.Ruffino
  7. Exp. Cell Res. v.185 Intracelluar metallothionein concentration and the rate of zinc or cadmium influx and MT mRNA accumulation in a CHO Cdr variant Morris,S.;P.C.Huang
  8. Br. J. Exp pathol. v.62 A morphological and histochemical study of the effects of L-cysteine on the renal uptake and nephrotoxicity of cadmium Murakami,M.;M.Webb
  9. Biochemstry v.10 Pulmonary Alveolar Macrophage Oxidative metabolism of isolated cells and mitochondria and effects of cadmium ion on election and energy transfer reaction Mustafa,M.G.;C.E.Cross
  10. Normal table of Xenopus laevis (Daudin) Nieuwkoop,P.D.;J.Faber
  11. Scand J Work Environ Health v.12 Airborne cadmium and carcinogenesis of the respitory tract Oberdorster G.
  12. Experientia v.42 Teratogenic effects of cadmium on Bufo arenarium during gastrulation Perez,C.S.;J.Herkovits;A.Salibian
  13. Teratogen. Carcinogen. Mutagen Teratogenicity of cobalt chloride in Xenopus laevis, assayed by the FETAX procedure Plowman,M.C.;Peracha,H.;Hopfer,S.M.;Sunderman,F.W.,Jr.
  14. Res. Board Can. v.33 Rosenthal,H.;Alderdice,D.F.;J.Fish
  15. Biol. Metals v.2 Uptake and release of $Ni^{2+}$ by Xenopus embryos during early cleavage stages Sunderman,F.W.,Jr.;Mongillo,F.J.;Plowman,M.C.;Brenan,S.M.
  16. An. Rev. Biochem. v.41 Biochemical effects of mercury, cadmium, a lead. Valle,B.L.;D.D.Ulmer
  17. Metal carcinogenesis Cadmium Carcinogenesis Waalkes,M.P.;Oberdorster,G.;Foulkes,E.C.(ed.)