Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Endothelial Cell Seeding Onto the Extracellular Matrix of Fibroblasts for the Developement of Small Diameter Polyurethane Vessel

소구경 폴리우레탄 인공혈관의 개발을 위한 세포외기질위의 혈관내피세포 배양

  • 박동국 (단국대학교 의과대학 일반외과학교실) ;
  • 이윤신 (서울대학교 의과대학 의공학연구소, 순천향대학 부속병원 성형외과학교실, 서울대학교 외과대학 병리학교실)
  • Published : 1995.03.01
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Abstract

A variety of experiments of endothelial cell seeding onto artificial vessels have been performed. To improve endothelialization, one or two components of the extracellular matrix (ECM) have been used as an underlying matrix. In this study, the whole ECM excreted from fibroblasts was used as an underlying matrix. Fetal human fibroblasts were cultured on a polyurethane (PU) sheet. After a conflu; ence was attained, the cytoskeleton and the nuclei of the fibroblast were destroyed using Triton-X. Mitomycin, or irradiation. Omental microvascular endothelial cells from adult human were seeded onto various substrates. After 12 days in culture, the cells were counted. It was observed that the ECM treated by irradiation had the highest cell number. In addition, the cells on this substrate exhibited the most typical endothelial cell morphology. For preliminary animal experiments the PU vessels (inner diameter, 1.5mm) coated with ECM were implanted in the infrarena] abdominal aorta of rat. After the vessels had been implanted for 5 weeks, it was found that the surface of the PU vessels was completely covered with endothelia] cells. In conclusion, we can state that the fibroblast-derived whole ECM makes a better underlying substrate for the endothelialization of small diameter artificial vessels.

Keywords

References

  1. Surgerry v.95 Reduced thrombogenic charactristics of espanded polytetra-fluoroethylene and polyurethane arterial grafts after heparin bonding Bjorek,C.G.;Esquivel,C.O.;Bergentz,C.E.(et al.)
  2. Nature v.275 Fibronectins-adhesive glycoproteins of cell surface and blood Yamada,K.M.;Olden,K.
  3. ASAIO Trans v.28 Endothelial cell seeding of small diameter vascular grafts Belden,T.A.;Schmit,S.P.;Falkow,L.I.;Sharp,W.V.
  4. ASAIO Trans v.34 New graft material and current approaches to an acceptable small diameter vascular graft Anson,Y.;Callow,A.D.
  5. J Vasc Sung v.6 Endothelial seeding: Problems and expectation Callow,A.D.
  6. Surgery v.101 vitro endothelialization ofsmall caliber vasculqar grafts Anderson,J.S.;Price,T.M.;Hanson,S.R.;Hanker,L.A.
  7. Pathobilogy of the endothelial cell Nossel,H.L;Vogel,H.J.
  8. Biochem J v.186 The effect of ascorbic acid on the nature and production collagen and elastin by rat smooth muscle cells Declerck,Y.A.;Jones,P.A.
  9. J Cell Biol v.81 Isolation of the pericellullar matrix of human fibro-blast cultures Hedman,K.;Kurkinen,M.;Alitalo,K.;Vaheri,A.;Johansson,S.;Hook,M.
  10. Arterioscierosis v.1 Secretory phenorypes of endothelial cells in culture: comparison of aortic, venous capillary and corneal endothelium Sage,H.;Pritzl,P.;Bornstein,P.
  11. KOSOMBE v.12 Endothelial cell seeding onto extracellular matrix for development of palyurehame vascular prosthesis Lee,Y.S.;Park,D.K.;Min,B.G.
  12. J Clin Invest v.71 Isolation and culture of microvascular endothelium from human adipose tissue Kern,P.A.;Kedler,A.;Eckel,R.H.
  13. vitro cellular development biology v.23 Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion Ingber,D.E.;Mardi,J.A.;Folkman,J.
  14. Cytotechnology v.3 Comparative behaviour of L-929 fibroblast and human endothelial cell onto crosslinked protein substrates Warocquier-Clerout R.;Lee,Y.,S.;Penhoat,J.;Sigot-Lulzard M.F.
  15. Thesis of Master, Seoul National University Antithrombogenic effect of endothelial cells cultured on extracellular matrix coated polywrethane; prostaglandin synthesis and platelet adhesion studies Myung A. Kim