약학회지 (YAKHAK HOEJI)

  • 제39권5호
  • /
  • Pages.516-520
  • /
  • 1995
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

항 단실 항체의 카르보닐탄소 유래 시그날의 귀속

Assignment of the Carbonyl Carbon Resonances in Anti-Dansyl Antibodies

  • ;
  • ;
  • Koichi Kato (Faculty of Pharamaceutical Sciences, University of Tokyo) ;
  • Yoji Arata (Faculty of Pharamaceutical Sciences, University of Tokyo)
  • 발행 : 1995.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The anti-dansyl antibodies were specifically labeled with stable isotope by growing hybridoma cells in serum-free medium. Assignments of the observed carbonyl carbon resonances have been determined by using $^{13}C-{15}N$ double labeling method in order to assign the Leu resonances. However, when the identical dipeptide appears more than twice in the polypeptide sequences, we applied the proteolytic fragments in the fragment-specific method. Carboxypep-tidase B-treated antibody has also been used to assign the Lys-447 in C terminal amino acid. These unambiguously assigned carbonyl carbon resonances in antibodies are thought to be useful in elucidating not only the structure of antibodies but also the structure-function relationship in the antibody by $^{13}C$ neuclear magnetic resonance spectroscopy.

키워드

참고문헌

  1. Nature(London) v.264 Crystallographic structure studies of an IgG molecule and an Fcfrgment Huber,H.;Deisenhofer,J.;Colman,P.M.;Matsushima,M.;Palm,W.
  2. Nature(London) v.360 The three-dimensional structure of an intact monoclonal antibody for canine lymphoma Harris,L.J.;Larson,S.B.;Hasel,K.W.;Day,J.;Greenwood,A.;McPherson,A.
  3. J. Mol. Biol. v.236 Dynamical structure of the hinge region of immunoglobulin G as studied by 13C nuclear magnetic resonances spectroscopy Kim,H.H.;Matsunaga,C.;Yoshino,A.;Kato,K.;Arata,Y.
  4. Biochemistry v.30 Complete assignment of the methionyl carbonyl carbon resonances in switch variant anti-dansyl antibodies labeled with [1-¹³C] Methionine Kato,K.;Matsunaga,C.;Igarashi,T.;Kim,H.H.;Odaka,A.;Shimada,I;Arata,Y.
  5. J. Immunol. Methods v.153 ¹³C-NMR spectral analysis of the structures of mouse immunoglobulin G1 carrying allotypes a and j Kim,H.H.;Kato,K.;Higuchi,A.;Nomura,N.;Noguchi,H.;Arata,Y.
  6. J. Biol. Chem. v.269 O-glycosylation in hinge region of mouse immunoglobulin G2b Kim,H.H.;Yamaguchi,Y.;Masuda,K.;Mastnunaga,C.;Yamamoto,K.;Irimura,T.;Takahashi,N.;Kato,K.;Arata,Y.
  7. Structure v.15 Model for the complex between protein G and an antibody Fc fragment in solution Kato,K.;Lian,L.;Barsukov,I.L.;Derrick,J.P.;Kim,H.H.;Tanaka,R.;Yoshino,A.;Shiraishi,M.;Shimada,I.;Arata,Y.;Roberts,G.C.K.
  8. FEBS Lett. v.346 Application of ¹³C-NMR spectroscopy to paratope mapping for large antigen-Fab complexes Kim,H.H.;Kato,K.;Yamato,S.;Igarashi,T.;Mastunaga,C.;Ohtsuka,H.;Higuchi,A.;Nomura,N.;Noguchi,H.;Arata,Y.
  9. Biochemistry v.21 Assignment of the three methionyl carbonyl resonances in Streptomyces subtilisin inhibitor by a carbon-13 and nitrogen-15 double-labeling technique. A new strategy for structural studies of proteins in solution Kainosho,M.;Tsuji,T.
  10. Biochemistry v.29 Structure of a mouse immunoglobulin G that lacks the entire Cн1 domain: protein sequencing and small-angle x-ray scattering studies Igarashi,T.;Sato,M.;Kastube,Y.;Takio,K.;Tanaka,T.;Nakanishi,M.;Arata,Y.
  11. Nature(London) v.307 Correlation between segmental flexibility and effector function of antibodies Oi,V.T.;Vuong,T.M.;Hardy,R.;Reidler,J.;Dangle,J.;Herzenberg,L.A.;Stryer,L.
  12. J. Immunol. Methods v.181 Proteolytic fragmentation with high specificity of mouse immunoglobulin G Yamaguchi,Y.;Kim,H.H.;Kato,K.;Masuda,K.;Shimada,I.;Arata,Y.
  13. Nature(London) v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  14. Rev. Sci. Instrum. v.29 Modified spin-echo method for measuring nuclear times Meiboom,S.;Gill,D.
  15. J. Mol. Biol. v.141 Crystallographic refinement and atomic models of the intact immunoglibulin molecule Kol and its antigen-binding fragment at 3.0 Å and 1.9Å resolution Marquart,M.;Deisenhofer,J.;Huber,R.;Palm,W.
  16. Biochemistry v.20 Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9Å and 2.8Å resolution Deisenhofer,J.
  17. Nature(London) v.332 Localization of the binding site for the human high-affinity Fc receptor on IgG Duncan,A.R.;Woof,J.M.;Patridge,L.J.;Burton,D.R.;Winter,G.