Effects of Spermine on Changes in Chlorophyll-Protein Complexes and Plastic Membrane Proteins of Mung Bean Cotyledons during Greening

녹화중인 녹두 자엽의 엽록소-단백질 복합체 및 색소체막 단백질의 변화에 미치는 Spermine의 효과

Developmental changes of chlorophyll-protein complexes (CPs) and plastid membrane proteins in greening mung bean cotyledons and the effect of spermine therein were examined by SDS-polyacrylamide gel electrophoresis. The changes in the amounts of CPs became larger with the progress of greening and light-harvesting chlorophyll a/b protein (LHCP) was the main CP in the early greening stage up to f h. As the greening proceeded, chlorophyll-protein of the photosystem I (CPI) accumulated. Application of spermine were effective in accumulating CPs of the thylakoid membrane in the early phase of greening. In the profiles of the plastid membrane proteins, quantitative and qualitative changes were observed with the onset of greening up to 72 h. 56 kD protein of major intensity was observed in all greened chloroplasts and 24 kD protein increased remarkablly in both control and spermine-treated cotyledons. The thylakoids from spermine-treated cotyledons showed hither amounts of thylakoid proteins as compared to the controls. The results suggest that spermine may play a role in the regulation of plastid development and stabilizes the membrane function during greening.

Spermine이 녹화중인 녹두자엽의 엽록소-단백질 복합체(CPs) 및 틸라코이드막 단백질의 변화에 미치는 효과를 조사하였다. 녹화가 진행됨에 따라 Cps형성이 촉진되었으며, 특히 광계의 엽록소-단백질(CP I)이 다량 추척되었다. 광수화 엽록소 단백질(LHCP)은 48시간의 초기 녹화과정에서 중요한 단백질로 나타났다.Spermine처리는 초기녹화과정에서 틸라코이드막의 CPs 축척에 효과적이었다. 색소체막 단백질은 녹화과정에서 많은 변화를 나타내었는데, 56kD단백질은 전 엽록체체서 다량 관찰되었꼬 24kD 단백질은 전 처리구에서 뚜렷한 증가를 보여주었다.Spermine처리에 의해 틸라코이드막 단백질 형성은 대조구에 비해 다소 증가되었다. 이러한 결과들로부터 spermine은 녹화과정에서 색소체의 발달과 색소체막의 안정화에 중요한 작용을 하는 것으로 생각된다.