Journal of fish pathology (한국어병학회지)
- Volume 8 Issue 2
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- Pages.91-98
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- 1995
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
Characterization of birnavirus isolated from cultured flounder fry
양식 넙치 치어에서 분리한 birnavirus의 특성
- Sohn, Sang-Gyu (Pathology Division, National Fisheries Research and Development Agency) ;
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Park, Myoung-Ae
(Pathology Division, National Fisheries Research and Development Agency) ;
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Do, Jeong-Wan
(Department of Microbiology, University of Ulsan) ;
- Jung, Cho-Rok (Pathology Division, National Fisheries Research and Development Agency) ;
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Park, Jeong-Woo
(Department of Microbiology, University of Ulsan)
- Published : 1995.12.30
Abstract
During 1993 and 1994, some mortalities of flounder(Paralichthy olivaceus) fry were recorded in several fish farms and viruses were isolated from 3 of the farms. Electron microscopic examination revealed that the virus particles were hexagonal and unenveloped with an average diameter of 50 to 55nm. Serological and molecular properties of these isolates were examined. The viral RNA and polypeptides patterns on electrophoresis, as well as neutralization test results, showed that these isolates were birnaviruses and two were closely related to infectious pancreatic necrosis virus(IPNV) serotype AB and one was to IPNV serotype SP. This is the first isolation of birnaviruses from marine fish in Korea.
최근 남해안 일대의 육상 양식장에서 사육중이던 넙치(Paralichthy olivaceus) 치어가 폐사하여 조사한 결과 3개 양식장에서 바이러스가 분리되었다. 분리된 바이러스들은 모두 외막이 없는 정육면체 모양이었으며 50~55mm 정도의 크기를 지녔다. 전기영동상에서 RNA와 구조 단백질의 patterns를 확인하고, IPNV에 대한 항혈청을 사용하여 중화실험을 수행한 결과, 분리한 세 바이러스는 birnavirus인 IPNV와 매우 유사함이 밝혀졌다. 특히 분리 바이러스중 CS는 IPNV의 AB 혈청형과 DS와 YJ는 SP 혈청형과 유사하였다.