Inhibition of Listeria monocytogenes by Bacteriocin(s) from Lactic Acid Bacteria Isolated from Kimchi

김치에서 분리한 젖산균 bacteriocin에 의한 Listeria monocytogenes의 억제

Four strains of lactic acid bacteria which produced bacteriocins inhibitory to Listeria species were isolated from Kimchi, and were identified as Leuconostoc mesenteroides subsp. mesenteroides (2 strains), Leuconostoc paramesenteroides and Pediococcus pentosaceus. The bacteriocins produced by the isolates inhibited all of the Listeria monocytogenes strains tested, but L. denigrificans 28 and L. welchimeri 89 were not inhibited by the bacteriocin produced by the Leu. paramesenteroides isolate. The bacteriocin produced by the P. pentosaceus isolate was more inhibitory against sensitive strains and showed broader spectrum of antimicrobial activity than those produced by other isolates. The bacteriocins produced by Leuconostoc isolates were sensitive to pronase E treatment, but that produced by the P. pentosaceus isolate was not completely inactivated. The bacteriocins produced by all of the isolates were not sensitive to catalase, ${\alpha}$-amylase and lysozyme and heat (30 min at $100^{\circ}C$) treatments.

김치로부터 Listeria spp.를 억제하는 박테리오신을 생산하는 젖산균 4균주를 선발하여 Leuconostoc mesenteroides subsp. mesenteroides (2균주), Leuconostoc paramesenteroides 및 Pediococcus pentosaceus로 동정하였다. 실험한 모든 Listeria monocytogenes 균주들은 모든 분리 균주의 박테리오신에 의해 억제되었으나, L. denigrificans 28과 L. welchimeri 89는 Leu. paramesenteroides 분리균주에 의해 억제되지 않았다. 분리균주들 중에서 P. pentosaceus의 박테리오신이 가장 항균활성이 높았고, 항균범위도 넓었다. Leuconostoc 분리균주들의 박테리오신의 항균활성은 pronase E에 의해 완전히 불활성화 되었으나 P. pentosaceus의 것은 완전히 불활성화되지 않았다. 분리균주들의 박테리오신은 catalase, ${\alpha}$-amylase, lysozyme 및 $100^{\circ}C$ 30분의 열처리에 대해서 안정하였다.