The Korean Journal of Physiology
- 제29권2호
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- Pages.243-250
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- 1995
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- 0300-4015(pISSN)
Interaction between Cholecystokinin and Secretin in Isolated Rat Pancreatic Acini
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Yoon, Shin-Hee
(Department of Physiology, Catholic University Medical College) ;
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Hahn, Sang-June
(Department of Physiology, Catholic University Medical College) ;
- Sim, Sang-Soo (Department of Physiology, Catholic University Medical College) ;
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Rhie, Duck-Joo
(Department of Physiology, Catholic University Medical College) ;
- Song, In-Young (Department of Physiology, Catholic University Medical College) ;
- Baek, Hye-Jung (Department of Physiology, Catholic University Medical College) ;
- Kim, Myung-Suk (Department of Physiology, Catholic University Medical College) ;
- Jo, Yang-Hyeok (Department of Physiology, Catholic University Medical College)
- 발행 : 1995.12.30
초록
A possible potentiation between cholecystokinin (CCK) and secretin in amylase secretion from isolated rat pancreatic acini was investigated. Combined treatment of acini with secretin and CCK at low concentrations, which are known to be physiological, resulted in enzyme secretion larger than the arithmetic sum of their separate effects. Such a potentiating effect also occurred between secretin and A23187 (Ca ionophore), between forskolin (adenylate cyclase activator) and CCK, and between forskolin and A23187. Staurosporin (protein kinase C inhibitor) and W7 (calmodulin antagonist) inhibited markedly the potentiated amylase release induced by the agonists, but KT5720 (protein kinase A inhibitor) did not affect the potentiated amylase release. Therefore, we concluded that the action of CCK in a physiological concentration is potentiated by secretin in a physiological concentration range and vice versa, and that the intracellular mechanism necessary for the potentiation is associated with