Journal of Aquaculture (한국양식학회지)
- Volume 7 Issue 3
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- Pages.159-164
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- 1994
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- 1226-0193(pISSN)
Production of All-Female Diploid and Triploid Populations in Paralichthys olivaceus
전 암컷 2배체 및 3배체 넙치(Paralichthys olivaceus)의 생산
- KIM Dong Soo (Department of Aquaculture, National Fisheries University of Pusan) ;
- MOON Young Bong (National Fisheries Research and Development Agency) ;
- JEONG Chang Hwa (Department of Aquaculture, National Fisheries University of Pusan) ;
- KIM Bong-Seok (Department of Marine Biology, National Fisheries University of Pusan) ;
- LEE Young-Don (Marine Research Institute, Cheju National University)
- Published : 1994.08.01
Abstract
All-female diploid Paralichtlrys olivaceus populations were produced by the artificial fertilization with the normal eggs and gynogenetic diploid males sperms. All-female triploids were also conducted to the fertilized eggs by cold shock. Floating, fertilization and hatching rates of the all-female diploid eggs were not significantly different from that of the diploid control eggs (P>0.05). All-female triploids were also not significantly different from their diploid controls or all-female diploid groups (P>0.05) in the floating and fertilization rates of eggs. However, hatching rates of all-female triploid groups were lower than that of the control (P<0.05). Induction of the triploids were confirmed by the measurement of erythrocyte sizes and by the chromosome counts. The volumes of erythrocytes and nucleus of triploid were larger than those of diploids, respectively. Percent incidences of triploid were
넙치 암컷과 유도된 자성발생성 2배체 숫컷 넙치를 교배시켜 넙치의 전 암컷 2배체를 생산한 후, 전 암컷 수정난에 저온처리하여 전 암컷 3배체 넙치를 생산하였다. 그 결과 부상율 및 수정율에 있어서 2배체 대조군, 전 암컷 2배체, 3배체 대조군 그리고 전 암컷 ,3배체군 모두에서 차이가 없었다 (P>0.05). 그러나, 3배체 대조군 및 전 암컷 3배체군은 여타 실험군에 비해 부화율이 낮게 나타났다 (P<0.05). 생식소의 조직학적 분석 결과 전 암컷 2배체 처리군 및 전 암컷 3배체 처리군의 성비는 모두
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