Decolorization of Poly R-478 Dye by Coriolus versicolor IFO 30388

구름버섯(Coriolus versicolor IFO 30388)에 의한 Poly R-478 염료의 탈색

Effects of nitrogen and carbon sources on the decolorization rate of poly R-478 dye by a white rot basidiomycete Coriorus versicolor IFO 30388 were examined. The fungus exhibited 87.2% of decolorization rate when it was cultured in the state of stationary in a nitrogen-limited medium (pH 4.5) which contained 2.0% glucose, 0.04% ammonium tartrate, 0.02% poly R-478 dye, 2% $KH_2PO_4$, 0.5% $MgSO_4{\cdot}7H_2O$, 0.1% $CaCl_2{\cdot}2H_2O$, 0.002% thiamine-HCl and 10 mM 2,2 dimethylsuccinate (sodium) at $28^{\circ}C$ for 10 days. Decolorization of the dye occurred in the presence of nitrogen source in the medium and decolorization rate increased rapidly after depletion of $NH_4^+$ from the medium.

구름버섯에 의한 Poly R-478염료의 탈색에 미치는 탄소원과 질소원의 영향을 조사하였다. 2.0% glucose, 0.04% ammonium tartrate, 2% $KH_2PO_4$, 0.5% $MgSO_4{\cdot}7H_2O$, 0.1% $CaCl_2{\cdot}2H_2O$, 0.002% thiamine-HCI, 10 mM 2,2-dimethylsuccinate(sodium)와 0.02% Poly R-478 염료 등으로 조성된 질소제한배지에서 균주를 $28^{\circ}C$에서 10일간 정치배양 했을때 염료의 탈색율은 87.2%로 나타났다. 염료의 탈색은 배지내에 질소원이 존재할 때에 일어났으며 탈색율은 배지내에 질소원이 고갈된 직후에 급격히 증가하였다.