Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Effects of Media, Hormones, and PFP on the Proliferation of Red Callus in Leaf Tissue Cultures of Garden Orach(Atriplex hortensis L.)

Garden Orach 조직배양에서 적색 캘러스의 증식에 미치는 배지, 호르몬 및 PFP의 영향

  • Published : 1994.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of medium, hormones, and PFP on the proliferation of red callus in leaf tissue cultures of Garden orach(Atriplex hortensis L.) was investigated. As a result,88% of leaf tissues formed eallus on MS nledium containing 2mg/$\ell$ 2,4-D. Fresh weight of callus was higher on MS medium than on Bsand NN media. It was also found that 2, 4-D was more effective than Dicamba and Picloram. The op-timum concentrations of hormones for callus proliferation depended on culture media. Isolated red cal-lus grew markedly both on MS medium supplemented with 1-2mg/$\ell$ 2, 4-D and Bs medium contain-ing 2-4111g/$\ell$ 2,4D. Callus proliferated on B5 and NN media containing Dicabma Img/$\ell$ as well as onthe same media containing 2mg/$\ell$ Picioram. The addition of PFP concentrations of 2, 5, and 40mg/ $\ell$rcspectiely to culture medium caused increase of callus fresh weight, especially under light condition.

Keywords

References

  1. Plant Cell Tissue & Organ Culture v.12 Induction of embryogenic Triticum aestivum L. Calli.Ⅱ. Quantification of genetic addenda and other culture variable effects Carman,J.M.;Jefferson,N.E.;Campbell,W.F.
  2. Enviro. Exp. Bot. v.22 Influence of different auxins at varying concentrations on callus induction and growth from embryo and leaf tip explants in Graminiae Conger,B.V.;Hileki,L.L.;Lowe,K.W.;Carabia,J.V.
  3. Plant Cell. Tissue and Organ Culture v.27 Effects of high ammonium concentrations on growth and anthocyanin fromation in grape(Vitis vinifera L.) cell suspension cultured in a production medium Do Chi Bao;Carmier Francois
  4. Plant Cell. Tissue and Organ culture v.20 Comparison of 2, 4-D and picloram for selection of long term totipotent green callus cultures of sugarcane Fitch,M.M.;Moore,P.U.
  5. Plant Cell Reports v.6 Clonal variability and light induction of betalain synthesis in red beet cell cultures Girod,P.A.;Zryd,J.P.
  6. 강원대 농업과학연구소 논문집 v.4 PFP(Para-fluorophenylalanine)처리에 의한 담배(Nicotiana tabacum L.) 배양조직의 유전적 변이 홍순관;신영범
  7. 대한식물도감 이창복
  8. 식물색소-實驗, 硏究 手引 林孝三
  9. 식물조직배양학회지 v.20 no.3 Red beet(Beta vulgaris L.)의 모상근 배양에 의한 betalain생산 백윤웅;안준철;정병균;김수웅;황백
  10. 식물조직배양학회지 v.21 no.2 꽃기린(Euphorbia Splendens Bojar) 배양세포로 부터 화청소의 대량생산 및 동정 선정훈;정재동
  11. 한육지 v.25 no.2 쪽의 청색색소 생산을 위한 캘러스 유기와 배양 소은희;채영암
  12. 한육지 v.23 no.2 Para-fluorophenylalanine(PFP)처리에 의한 작물의 염색체 변이,Ⅰ. 배지, PFP 및 호르몬이 메밀 및 담배 callus 유기 및 생장에 미치는 영향 신영범
  13. 世界有用植物事典 堀田 滿