Effects of Dietary Irons Supplementation on ${\delta}-Aminolevulinic$ Acid Dehydratase Activity and Detoxification of the Pb-administered Rats

철보충이 납중독된 흰쥐의 ${\delta}-Aminolevulinic$ Acid Dehydratase 활성과 해독에 미치는 영향

  • 이정숙 (고신대학교 식품영양학과) ;
  • 조수열 (영남대학교 식품영양학과) ;
  • 김영로 (영남대학교 환경학과)
  • Published : 1994.02.01

Abstract

This study was designed to investigate the effects of dietary iron supplementationon ${\delta}-aminolevulinic$ acid dehydratase (DALAD) activity and liver damage of the lead(Pb)-administered rats. The iron (Fe) supplement levels were 100 ppm(Fe100) and 500ppm(Fe500) and the Pb-exposed rats were given 2, 000ppm-Pb in drinking water, while control rats were given neigher iron nor lead. Hematocrit was lower in the Pb, Fe100 -Pb, Fe500-Pb group than in the control, but was not affected by the Pb adminstration when the rats fed the Fe supplementation diet. DALAD activity were reduced by Pb added but, were higher in the Fe500-Pb group than in the Fe100 -Pb group. No significant difference was found in serum Pb content due to Pb administration and Fe supplementation. The liver Pb contentwas higher in the Fe supplementation group than in the Pb-group. Level of serum FE was lower in the Pb added groups than in the control group. Liver Fe contents were increased with Pb administration and higher in the Fe supplement groups than in the Pb-group. Levels of serum and liver copper was decreased with the Fe supplementation. Aminotransferase activity of serum and liver were increased in the Pb group.

철보충이 납종독된 흰쥐의 체내 무기질 함량과 DALAD activity 및 해독에 미치는 영향을 관찰하기 위해, 흰쥐에게 납 2,000ppm을 함유한 식수를 제한없이 주면서, 철분 100ppm, 500ppm을 보충한 식이로 5주동안 사육하여 얻을 결과는 다음과 같다. 체중증가량, 식이섭취량 및 식이효율은 납급여군이 대조군보다 낮게 나타났으며, 철보충납급여군은 납단독급여군보다 높은 것으로 나타났다. 간과 심장의 무게는 차이를 보이지 않았으나, 신장은 납급여군이 대조군보다 증가를 보였다. 비장은 납단독급여군에서만 증가를 보였고, 철보충납급여군에서는 대조군과 차이를 보이지 않았다. 헤마토크릿치는 철보충납급여군에서는 대조군과 차이를 보이지 않았으나, 납단독급여군은 유의적인 감소를 보였다. DALAD activity는 혈액과 간 모두 납급여군에서 대조군보다 유의적인 감소를 보였으나, 납단독급여군보다 철보충납급여군이 높게 나타났고, 철보충수준이 높을수록 높게 나타났다. 혈청과 간의 amino-transferase activity는 철보충납급여군에서는 대조군과 비슷한 경향을 보였으나 납단독급여군에서는 유의적인 증가를 보였다. 혈청 납함량은 납단독급여군과 철보충납급여군 사이에 차이를 보이지 않았으나, 간의 납함량은 철보충납급여군이 납단독급여군보다 높게 나타났다. 혈청 철함량은 납급여군이 대조군보다 감소했으나, 간의 철함량은 납급여군에서 높게 나타났으며, 철보충납급여군이 납단독급여군보다 증가를 보였다. 혈청과 간의 구리함량은 납단독급여군은 대조군과의 차이를 보이지 않았으나, 철보충납급여군은 감소를 보였다.

Keywords

References

  1. Medicine v.4 Lead poisoning Aub,J.C.;Fairhall,L.T.;Minot,A.S.;Reznikoff,P.
  2. Brit. J. Ind. Med. v.32 The effects of metal ions of δ-aminolevulinic acid dehydratase Thompson,J.J.;Beasley,W.H.
  3. Toxicol. Appl. Pharmacol. v.41 Influence of dietary factors of blood tissue lead Augusta,A.M.;Lornald,M.;Uthman,E.
  4. J. Pharm. Sci. Korea v.19 납중독에 있어서 δ-aminolevlinic acid dehydratase의 효과에 관한 연구 윤혜정
  5. J. Lab. Clin. Med. v.79 The influence of iron deficiency on tissue content and toxicity of ingested lead in the rat Six,K.M.;Goyer,R.A.
  6. Nutrition and environmental health Edward,J.C.
  7. J. Nutr. v.106 Influence of dietary selenium on lead toxicity in the rat Cerklewski,F.N.;Forbes,R.M.
  8. J. Med. v.284 δ-aminolevulinic acid dehydratase activity in circulating blood cells Weissberg,J.B.;Lipschutz,F.;Oski,F.
  9. Am. J. Clin. Pathol. v.28 A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase Reitman,S.;Frankel,S.
  10. J.A.O.A.C. v.51 Mineral in feeds by atomic absorption spectrophotometry Heckman,M.
  11. Principles procedures of statistics Steel,R.G.;Torrie,J.H.
  12. 한국영양학회지 v.19 no.4 단백질 급원과 수준을 달리한 식이가 흰쥐의 납 축적에 미치는 영향 김옥경;서정숙;이명환
  13. Toxicol. Appl. Pharmacol. v.41 Influence of dietary factors on blood and tissue lead concentrations and lead toxicity Mylroie,A.A.;Moore,L.;Uthman,E.
  14. Pediat. Res. v.11 Experimental lead poisoning and intestinal transport of glucose, amino acid and sodium Wapnir,R.A.;Exeni,R.A.;Mcvicar,M.;Lifsheitz,F.
  15. Environ. Res. v.12 The effects of dietary lead content and food restriction on lead retention in rats Quarterman,J.;Morrison,J.N.;Humphries,W.R.
  16. J. Nutr. v.109 Effect of dietary supplementaion of iron and ascorbic acid on lead toxicity in rats Suzuki,T.;Yoshida,A.
  17. 한국영양학회지 v.18 no.4 유년기 백서의 단백질 부족이 성장후 납중독에 미치는 영향 김양선;유정열
  18. J. Nutr. v.107 Anemia of lead intoxication : A role for copper Klauder,D.S.;Petering,H.G.
  19. Nutr. Rev. v.34 no.11 Recommendations for the prevention of lead poisoning in children Chisolm,J.J.
  20. Lead effects on the heme biosynthetic pathway Moore,M.R.;Goldberg,A.;Yeung-Laiwah,A.A.
  21. Scientic American v.224 Lead poisoning Chisolm,J.J.
  22. 중대논문집 납중독에 있어서 고려 인삼근 수침액의 해독효과에 관한 연구 ( I ) 김윤영
  23. Arch. Biochem. Biophys. v.144 Biochemical effects of mercury, cadmium and lead Vallee,B.L.;Ulmer,D.D.
  24. Gastroenterology v.74 Factors affecting the absorption and excretion of lead in the rat Conrad,M.E.;Barton,J.C.
  25. J. Biol. Chem. v.239 The biochemistry of animals cells, the effect of corticosteroids of leakage of enzyme from dispersed rat liver cells Takeda,Y.;Ichihara,A.;Tanioka,H.;Inove,H.
  26. Behavioral Sciences and Health Care v.1 Blood lead of iron deficient children-increase following iron supplementation Carol,R.;Matilda,S.M.
  27. Nephron v.2 The anemia of lead poisoning Galle,P.;Morel-Marager,L.
  28. J. Nutr. v.107 Influence of dietary copper on lead toxicity in the young male rat Cerklewski,F.L.;Forbes,R.M.