한국수정란이식학회지 (Journal of Embryo Transfer)
- 제9권3호
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- Pages.221-227
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- 1994
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
소 체외수정란의 체외배양 및 이식후 생존성
Viability of In Vitro Fertilized Bovine Embryos Following In Vitro Culture and Embryo Transfer
초록
This study was conducted to examine the condition of in vitro culture system and the viability after embryo transfer of in vitro matured-in vitro fertilized (IVM-IVF) bovine embryos. The in vitro development to the blastocyst stage was enhanced by supplying bovine serum albumin(BSA) to co-culture medium with bovine oviduct epithelial tissue(BOET) compared with that in medium supplemented with fetal bovine serum(FBS) (41.2% vs. 26. 3%, P<0.05). After transfer of IVM-IVF blastocysts into the uterine horn of recipient females (Aberdeen Angus), one was pregnant to term and produced a head of male Korean native calf. These results confirm that the in vitro development of IVM-IVF bovine embryos is affected with different protein source in co-culture with BOET, and IVM-IVF embryos can develop to term after in vitro culture and embryo transfer.
키워드