Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Improved Anthocyanin Production in Hairy Root Culture of Daucus carota by Fungal Elicitor

당근의 모상근 배양에서 Fungal Elicitor에 의한 Anthocyanin 생산의 향상

  • Kim, Chang-Heon (Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Seong-Woo (Department of Horticulture, Kyung Hee university) ;
  • Whang, Baik (Department of Biology, Chonnam National University) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University)
  • 김창헌 (경희대학교 자연과학대학 유전공학과) ;
  • 이승우 (경희대학교 산업대학 원예학과) ;
  • 황백 (전남대학교 자연과학대학 생물학과) ;
  • 정인식 (경희대학교 자연과학대학 유전공학과)
  • Published : 1994.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to improve anthocyanin production, effects of fungal elicitation in hairy root culture were investigated. fungal elicitor prepared from Fusarium moniliforme was the best in enhancement of anthocyanin production among the eight fungal elicitors tested. The optimum treat-ment time and concentration of treated elicitor for anthocyanin production were 12 hours and 3.28 mg carbohydrates per liter medium. Also, fungal elicitor was treated to hairy root culture in flat-bottomed fluidized-bed bioreactor. The anthocyanin production of elicited culture was enhanced 227% than non-treated.

Keywords

References

  1. J. Bacteriol. v.164 Molecular and genetic analysis of the transfered DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes White,F.F.;B.H.Taylor;G.A.Huffman;M.P.Gordon;F.W.Nester
  2. Biotechnol. Bioeng. Symp. v.17 Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots cultures Jung,G.;D.Tepger
  3. Plant Cell. Rep. v.8 High yield production of tropane alkaloids by hairy-root cultures of a Datura candida hybrid Christen,P.;M.F.Roberts;J.D.Phillipson;W.C.Evans
  4. Biotechnology in Agricultural Chemistry(ACS symposium series, No. 334) Use of plant cells and organ culture in the production of biological chemicals Flores,H.E.;LeBaron,H.M.(ed.);R.O.Mumma(ed.);R.C.Honeycutt(ed.);J.H.Duesting(ed.)
  5. Plant Sci. v.59 Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes Mano,Y.;H.Ohkawa;Y.Yamada
  6. Theor. Appl. Genet. v.71 A study of karyotypes and their alterations in cultured and Agrobacterium transformed roots of Lycopersicon peruvianum Mill Banerjee-Chattopadhyay,S.;A.M.Schwemmin;D.J.Schwemmin
  7. Phytochem. v.26 Increased secondary product formation in plant cell suspension cultures after treatment with a yeast carbohydrate preparation(elicitor) Funk,C.;K.Gugler;P.Brodelius
  8. Plant Cell Rep. v.12 Anti-inflammatory oleanane triterpenes from Tripterygium wilfordii cell suspension cultures by fungal elicitation Kutney,J.P.;M.D.Samija;G.M.Hewitt;E.C.Bugante;H.Gu
  9. Cell Culture and Somatic Cell Genetics of Plants, Vol. 4 Elicitation: methodology and aspects of application Eilert,U.;Vasil,I.K.(ed.);F.Constabel(ed.)
  10. Trends in Biotechnol. v.3 Eliciting secondary metabolism in plant cell culture DiCosmo,F.;M.Misawa
  11. Methods in Plant Biochemistry, Vol. 2 v.2 Monosaccharides: Ⅲ. colorimetric assays Sturgeon,R.J.;Dey,P.M.(ed.);J.B.Harborne(ed.)
  12. Arch. Biochem. Biophys. v.166 Light-induced changes of enzyme activities in parsley cell suspension cultures Zimmermann,A.;K.Hahlbrock