• 한국미생물·생명공학회지
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• 제22권4호
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• pp.395-400
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• 1994

In order to improve anthocyanin production, effects of fungal elicitation in hairy root culture were investigated. fungal elicitor prepared from Fusarium moniliforme was the best in enhancement of anthocyanin production among the eight fungal elicitors tested. The optimum treat-ment time and concentration of treated elicitor for anthocyanin production were 12 hours and 3.28 mg carbohydrates per liter medium. Also, fungal elicitor was treated to hairy root culture in flat-bottomed fluidized-bed bioreactor. The anthocyanin production of elicited culture was enhanced 227% than non-treated.

• Applied Biological Chemistry
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• 제37권4호
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• pp.237-242
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• 1994

유동층 생물반응기에서의 anthocyanin 생산을 위하여 당근의 모상근배양을 검토하여 보았다. 이 생물반응기에서의 모상근의 성장은 2.5배 증가되었지만 anthocyanin 생산은 낮았다. 그러나 유동층 생물반응기에서의 anthocyanin 생산은 fungal elicitor의 처리에 의해 2.3배 향상되었다.

• Mycobiology
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• 제51권4호
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• pp.230-238
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• 2023

Glabridin is a well-known active isoflavone found in the root of licorice (Glycyrrhiza glabra L.) that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytes cultures are the most important alternative methods for plant resources conservation and sustainable production of natural compounds, which has received much attention in recent decades. In the present study, an efficient culture condition was optimized for the biomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticola SBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licorice root extract (as an elicitor) were tested. The results showed that the highest and lowest biomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt (5.85 + 0.11 g/l), respectively. The medium culture PCB was produced the highest level of glabridin (7.26 ± 0.44 mg/l), while the lowest level (4.47 ± 0.02 mg/l) was obtained from the medium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51 mg/l) production were observed from the PCB medium adjusted with pH = 6, while the lowest value of both traits was obtained from the same medium with pH = 7. The highest production of total glabridin (10.85 ± 0.84 mg/l) was also obtained from the culture medium treated with 100 mg/l of the plant root extract. This information can be interestingly used for the commercialization of glabridin production for further industrial applications.

• 식물조직배양학회지
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• 제24권5호
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• pp.273-277
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• 1997

Rhizoctonia solani 추출물(elicitor)을 Hyoscyamus muticus 모상근배양에 처리하였을 때 sesquiterpene cyclase (SC)가 유도됨과 동시에 sesquiterpene화합물이 합성되어 배양액속에 축적하는 것으로 나타났다. 24시간 배양기간 동안 배양액으로부터 sesquiterpene을 추출ㆍ분리하여 배양할 경우, 추출ㆍ분리하지 않은 모상근배양보다 거의 두배의 함량이 생성되었다. Cyclase monoclonal antibody을 이용하여 immunoblot을 시도한 결과, 추출한 모상근배양과 추출하지 많은 모상근배양에서의 SC 절대량은 동일한 것으로 나타났으며, 효소활성도 현저한 차이가 없는 것으로 측정되었다. 이러한 결과는 terpenoid pathway에서 sesquiterpene 생합성이 이용가능한 기질의 량에 의해 조절되는 것으로 사료되며, feedback 조절은 sesquiterpene cyclase 효소에 앞서 일어나는 것으로 추정된다.