Parasites, Hosts and Diseases
- Volume 32 Issue 2
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- Pages.101-110
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- 1994
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Characterization of the high mannose asparagine-linked oligosaccharides synthesized by microfilariae of Dirofilaria immitis
심장사상충 자충이 합성한 high mannose asparagine-linked oligosaccharides의 분자화학적 분석
Abstract
This report describes the structures of high-mannose-type N-linked oligosaccharides in glycoproteins synthesized by the microfilariae of Diroflcrio immitis. Microfilariae of D. immitis were incubated in vitro in media containing 2-(3H) mannose to allow metabolic radiolabeling of the oligosaccharide moieties of newly synthesized glycoproteins. Glycopeptides were prepared from the radiolabeled glycoproteins by digestion with pronase and fractionation by chromatography on concanavalin A Sepharose. Thirty eight percent of 2- (3H) mannose incorporated into the microalariae of D. immitis glycopeptides was recovered in high mannose-type asparagine-linked oligosaccharides which were bound to the immobilized lectin. Upon treatment of 2-(3H) mannose labeled glycopeptides with endo - β- N- acetylglu co saminidase H , the high mannose type chains were released and their structures were determined by high performance liquid chromatography and exoglycosidase digestion. The major species of high mannose-type chains synthesized by microfilariae of D. immitis have the composition Man5GlcNAc2, Man6ClcNAc2, Man7GlcNAc2, and Man8GlcNAc2. Structural analyses indicate that these oligosaccharides are similar to high mannose-type chains synthesized by vertebrates.
심장사상충 자충이 합성한 당단백의 분지(분지) 역할을 하는 N-linked high mannose 타입 올리고당의 구조에 대한 조사를 수행하였다. 사상충 자충을 방사선 표식 2-[
Keywords
- Dirofilaria immitis;
- mannose;
- carbohydrates;
- oligosaccharides;
- microfilariae;
- metabolic labeling;
- HPLC;
- enzyme digestion