YAKHAK HOEJI (약학회지)
- Volume 38 Issue 5
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- Pages.608-613
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- 1994
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Isolation and purification of Cd-binding high molecular weight protein in rat liver(I)
흰쥐 간에서의 카드뮴 결합 고분자량 단백질의 분리 및 정제(I)
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Chun, Ki-Jung
(Korea Atomic Energy Research Institute)
;
-
Kim, Bong-Hee
(College of Pharmacy, Chung Nam National University)
- Published : 1994.10.30
Abstract
We isolated two Cd-binding high molecular weight proteins from intraperitoneally cadmium injected rat liver. Molecular weight of Cd-BP(I) purified from Sephacryl S-100 and DEAE Sepharose column chromatography and Cd-BP(II) purified from DEAE Sepharose column chromatography and Sulphonyl Sepharose column chromatography was 33,000 and 18,400, respectively. Alcohol dehydrogenase and alkaline phosphatase acitivities were not detected from two purified proteins.
Keywords
- Cd-binding high molecular weight proteins;
- Sephacryl S-100;
- DEAE Sepharose;
- Sulphonyl Sepharose;
- Column chromatography;
- Alcohol dehydrogenase;
- Alkaline phosphatase