약학회지 (YAKHAK HOEJI)
- 제38권5호
- /
- Pages.595-601
- /
- 1994
- /
- 0377-9556(pISSN)
- /
- 2383-9457(eISSN)
이종간 원형질체 융합을 이용한 acetaminophen 생산균주 개량
Strain Improvement by Interspecific Protoplast Fusion of Streptomyces griseus and Streptomyces hygroscopicus producing Acetaminophen
- Sohn, Yeo-Won (National Institute of Health) ;
- Jung, Dae-Young (National Institute of Health) ;
- Lee, Sang-Sup (College of Pharmacy, Seoul National University) ;
- Min, Hong-Ki (National Institute of Health)
- 발행 : 1994.10.30
초록
Acetaminophen, a widely used analgesic, can be formed by N-acetylation and p-hydroxylation of aniline. Interspecific protoplast fusion technique was used to get acetaminophen directly from aniline and to increase the productivity of acetaminophen. Three auxotrophic mutants were obtained from S. griseus(ATCC 13273) and S. hygroscopicus(KCTC 1089) by N-methyl-N'-nitro-N-nitrosoguanidine(NTG) treatment. Regeneration frequencies of S. griseus