Identification of Active Component Isolated from Croton tiglium and Coptis japonica Aqueous Mixture$(CP_2)$ and Studies of Its Cytotoxic Effect

파두와 황련의 수용성 혼합물$(CP_2)$부터 분리된 항암성분의 구조확인 및 세포독성에 대한 연구

Active fraction, $P_2$, was Isolated from natural anti-cancer drug, $CP_2$, by HPLC. We confirmed that $P_2$ includes most of the Isoguanosine and minor components, Berberine and other protoberberine alkaloids, by $^1H-NMR$ and $^{13}C-NMR$ and measured tile cytotoxicity of $P_2$ against various tumor cell-lines.' $P_2$ was very effective to all tumor cell-lines, especially to human colon cancer SNU-C2A$(ED_{50};\;24{\mu}g)$ and liver cancer HEP-3B$(ED_{50};\;27{\mu}g)$.