YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Biosynthesis of Nitric Oxide in Pancreatic Tissues

췌조직내 Nitric Oxide의 생합성

  • Kim, Yong-Kee (Department of Biochemistry, College of Pharmacy, Sung Kyun Kwan University) ;
  • Nam, Suk-Woo (Department of Biochemistry, College of Pharmacy, Sung Kyun Kwan University) ;
  • Park, Seung-Hee (Department of Biochemistry, College of Pharmacy, Sung Kyun Kwan University) ;
  • Yoo, Se-Geun (Department of Biochemistry, College of Pharmacy, Sung Kyun Kwan University) ;
  • Hong, Sung-Youl (Department of Genetic Engineering, College of Life Science and Natural Resources, Sung Kyun Kwan University) ;
  • Lee, Hyang-Woo (Department of Biochemistry, College of Pharmacy, Sung Kyun Kwan University)
  • Published : 1994.02.28
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Abstract

Nitric oxide(NO) synthase was identified and characterized by determining the L-citrulline formed in the NO-Arg pathway in pancreatic tissues. NO synthase activities in chicken pancreas were dependent upon the concentration of L-Arg which is the substrate molecule for the NO synthase, the amount of the enzyme protein used, and linearly on the incubation time. NO synthase in mouse pancreas was found to be constitutive, not induced by lipopolysaccharide treatment. In vitro NO synthase activities of chicken pancreas were inhibited 36%, 21%, 12% and 44% by $200\;{\mu}M$ of MMA, DMA, D'MA and NAME respectively. These results suggest the presence of NO and NO synthase in the pancreas.

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