Penicillium verruculosum으로부터 D-xylanase II의 정제 및 특성

Purification and Characterization of D-Xylanase II from Penicillium verruculosum

  • 발행 : 1993.12.01

초록

Xylanase(1, 4-beta-D-xylan xylanohydrolase` EC 3.2.1.8) II was purified from Penicillium verruculosum by using the techniques of two anion exchange chromatographies, and gel filtration. The molecular weight of this enzyme was about 22, 000 as determined by SDS-electrophoresis. The enzyme showed hydropytic activity toward xylan but did not catalyze hydrolysis of Rho-nitrophenyl-beta-D-xylopyranoside, Rho-nitrophenyl-beta-D-glucopyranoside, Rho-nitrophenyl-beta-D-cellobiopyranoside, and celluloses such as Avicel, cotton, filter paper, carboxymethylcellulose.

키워드

참고문헌

  1. Appl. Microbiol. Biotechnol. v.30 D-xylanase produced by Schizo phyllum radiatum Cavazzoni,V.;Manzoni,M.;Parini,C.;M.C.Bonferoni
  2. Hakkokokogaku v.60 Production of cellulolytic enzymes by Penicillium verruculosum Chung,K.C.;Kwawai,K.;Yashima,S.;Y.Eguchi
  3. 한국생화학회지 v.24 Penicillium verruculosum으로부터 Cellobiohydrolase의 정제 및 특성에 관한 연구 조남철;김강화;전순배;정기철
  4. 한국산업미생물학회지 v.25 Penicillium verruculosum으로부터 D-xylanase의 정제 및 특성 조남철;권수진;김강화;정기철
  5. 한국생화학회지 v.25 Penicillium verruculosum으로부터 β-xylosidase의 정제 및 특성 조남철;김강화
  6. 한국산업미생물학회지 v.15 섬유소분해효소 생산증진을 위한 Penicillium verruculosum의 균주 개량 정기철
  7. 한국산업미생물학회지 v.18 Penicillium verruculosum 의 Avicelase 생성에 대한 Cellobio Octaacetate와 Avicel 및 KC-flock의 영향 조남철;김강화;정기철;전순배
  8. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller,G.L.
  9. J. Biol. Chem. v.193 Protein measurement with Follin-Phenol reagent Lowry,O.H.;Rosebrough,M.J.;Farr,A.L.;R.J.Randall
  10. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K.
  11. Carbohyydrate analysis Oligosaccharides White,C.A.;J.F.kennedy;Chaplin,M.F.(ed.);J.F.Kennedy(ed.)
  12. Meth. Enzymol. v.160 Xylanase A of Schizophyllum commune Jurasek,L.;M.G.Paice
  13. Meth. Enzymol. v.160 Xylanase and β-Xylosidase of Trichoderma lignorum John,M.;J.Schmidt
  14. Meth. Enzymol. v.160 Xylanase of Streptomyees Yasui,T.;Marui,M.;Kusakabe,I.;K.Nakanishi
  15. Appl. Microbiol. Biotechnol. v.29 Production and characterization of xylanase from Thielaviopsis basicola Ghosh,V.K.;J.K.Deb
  16. Appl. Microbiol. Biotechnol. v.33 Characterization of a major xylanase purified from Lentinula edodes cultures grown on a commercial solid lignocellulosic substrate Mishra,C.;Forrester,I.T.;Kelley,B.D.;Burgess,R.R.;G.F.Leatham
  17. Appl. Microbiol. Biotechnol. v.32 β-xylanase produced by Auerobasidium pullulans CBS 58475 Dobberstein,J.;C.C.Emeis
  18. Meth. Enzymol. v.160 Xylanase of Malbranchea pulchella var. sulfurea Matsuo,M.;T.Yasui