한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권6호
  • /
  • Pages.542-548
  • /
  • 1993
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Bacillus stearothermophilus Acetyl Xylan Esterase 유전자의 크로닝과 Escherichia coli에서의 발현

Molecular Cloning and Expression of the Acetyl Xylan Esterase Gene of Bacillus stearothermophilus in Escherichia coli

  • 김인숙 (고려대학교 유전공학과) ;
  • 조쌍구 (고려대학교 유전공학과) ;
  • 최용진 (고려대학교 유전공학과)
  • 발행 : 1993.12.01
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초록

Bacillus stearothermophilus was shown to express multiple xylanolytic enzymes including acetyl xylan esterase. Genomic DNA of the strain partially digested with HindIII was ligated into the HindIII site of pBR322, and expressed in E. coli HB101 cells in order to clone the gene for acetyl xylan esterase. One transformant among 4000 screened formed a clear zone around its colony on the LB agar supplemented with 1.0% tributyrin. The functional clone harbored the recombinant plasmid pKMG5 with an insert of 5.1kb.

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