한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제21권5호
  • /
  • Pages.504-510
  • /
  • 1993
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

AcNPV 감염 조건이 Spodoptera frugiperda 21 세포에서의 재조합 단백질 생산에 미치는 영향

Effect of AcNPV Infection Conditions on Recombinant Protein Production in Spodoptera frugiperda 21 Cells

  • 발행 : 1993.10.01
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초록

The effect of AcNPV infection conditions such as serum concentration, pH, CaCl2, lysosomotropic agent, cell density at infection, agitation, aeration and nutritional supplementattion on recombinant protein production in Spodoptera frugiperda 21 cells was investigated using tissue culture flask, bottle and spinner flask. It was shown that serum, CaCl2, pH and cell density at infection affected recombinant production. The lysosomotropic agent did not significantly influence recombinant protein production.

키워드

참고문헌

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