Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Expression of $\beta$-l,4-Glucosidase Gene from Pseudomonas sp. in Escherichia coli and Bacillus subtilis

Pseudomonas sp. $\beta$-1,4-Glucosidase 유전자의 Esherichia coli와 Bacillus subtilis에의 Cloning 및 발현

  • 김양우 (경성대학교 공과대학 식품공학과) ;
  • 전성식 (경성대학교 공과대학 식품공학과) ;
  • 김석재 (경성대학교 공과대학 식품공학과) ;
  • 정영철 (진주전문대학 식품영양학과) ;
  • 성낙계 (경성대학교 공과대학 식품공학과)
  • Published : 1993.04.01
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Abstract

Fro the purpose of producing glouse from cellobiose or oligo saccharide and obtaining genetic information of beta-1,4-glucosidase gene, alpha beta-1,4-glucosidase gene of Pseudomonas sp. LBC505, potent cellulase complex and xylanase producing strain, was cloned in Esherichia coli and Bacillus subtilis into pUC19 and pBD64, respectively. Recombinant plasmid pGL1 contained 1.2kb EcoRI fragment was isolated from transformants forming blue color around colony on LB agar plate containing 20 ng/ml of 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside(X-glu) and ampicillin.

Keywords

References

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