Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Screening of Alkalophilic Bacillus sp. for Overproduction of Cyclodextrin Glucanotransferase and Its Enzymatic Properties

Cyclodextrin Glucanotransferase 고생산 호알칼리성 세균의 탐색과 분비 효소의 특성

  • 도은주 (경북대학교 유전공학과) ;
  • 박종부 (경북대학교 유전공학과) ;
  • 이용현 (경북대학교 유전공학과)
  • Published : 1993.04.01
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Abstract

An alkalophilic microorganism for overproduction of cyclodextrin glucanotransferase (CGTase) was newly isolated from hot-water spring soil, and identified as Bacillus firmus var. alkalophilus H609. The strain maintained stability during preservation and cultivation for the enzyme production, and produced significant amount of CGTase corresponding to the volumetric activity of 75 units/mL at 37C, initial pH of 11.2, and after 40 hours. The strain excreted several different proteins showing CGTase activity that catalyzed the formation of mainly beta-and Gamma-type cyclodextrin (ratio of 7:1) from soluble starch without accumulation of alpha-type. Other enzymatic properties were also investigated.

Keywords

References

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