Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Crude Oil-degrading Properties of Psychrotrophic Bacterium Acinetobacter calcoaceticus A1-1

저온성 세균 Acinetobacter calcoaceticus A1-1의 원유분해 특성

  • 윤희정 (숙명여자대학교 생물학과) ;
  • 김상진 (한국해양연구소해양미생물연구실) ;
  • 민경희 (숙명여자대학교 생물학과)
  • Published : 1993.02.01
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Abstract

To investigate the oil degrading properties of psychrotrophic bacterium Acinetobacter calcoaceticus Al-l the effects of environmental factors on this bacterium were studied. The optimal environmental conditions for cell growth rate and oil-emulsifying activity were as follows; temperature $15^{\circ}C$, pH 7.5, salt concentration 0- 3% and crude oil concentration 0.1%. Additionally the optimal concentration of Nand P source for cell growth rate and oil-emulsifying activity were 0.76 mM and 0.057 mM as $(NH_4)HS0_4$ and $K_2HP0_4$, respectively. Analysis of remnant oil by gas chromatography showed time dependent oil degradation pattern by A. calcoaceticus during cultivation; At lOoe and $15^{circ}$e, most alkane peaks were disappeared and it showed large quantities of crude oil were degraded. But at $25^{circ}$e alkane compounds in the crude oil were partially degraded even after 120 hours incubation.

저온성 세균 Acinetobacter calcoaceticus A1-1의 유류분해 특성을 연구하기 위해 환경적 요인에 대한 영향을 살펴보았다. 이 세균의 성장율과 유화활성도에 대한 적정 환경조건은 온도 $15^{\circ}C$, pH 7.5, 염분도 0-3, 원유농도 0.1로 나타났고 질소원과 인원의 적정농도는$(NH_4)_2S0_4$과 $K_2HP0_4$, 형태로 각각 0.76 mM, 0.057mM이다. 온도에 따른 A.calcoaceticus A1-1의 유류분해 양상을 개스크로마토그라피로 분석한 결과 $10^{\circ}C$, $15^{\circ}C$에서 대부분의 탄화수소 피크가 감소되어 상당 정도의 유류분해를 관찰 할 수 있었다. 반면 $25^{\circ}C$의 경우에는 120시간 배양한 후에도 원유에 포함된 포화탄화수소 화합물들이 부분적으로만 분해되었다.

Keywords

References

  1. 산업미생물학회지 v.21 저온성 원유분해세균의 분리동정 및 OCT 프라스미드 특성 김상진;윤희정
  2. Biotech. Bioeng. v.14 Degradation and mineralization of petroleum in sea water: limitation by nitrogen and phosphorous Atlas,R.M.;R.Bartha
  3. Environ. Pollut. v.4 Abundance, distribution and oil biodegradation potential of microorganisms in Raritan bay Atlas,R.M.;R.Bartha
  4. Appl. Microbiol. v.30 Effects of temperature and crude oil composition on petroleum biodegradation Atlas,R.M.
  5. Fates and effects of petroleum hydrocarbons in marine ecosystem and organisms Studies on petroleum biodegradation in the Arctic Atlas,R.M.;D.Wolfe(ed.)
  6. J. Fish. Res. Board Can. v.35 Prudhoe crude oil in arctic marine ice, water and sediment ecosystems: degradation and interactions with microbial and benthic communities Atlas,R.M.;A.Horowitz;M.Busdosh
  7. Microbiol. Rev. v.45 Microbial degradations of petroleum hydrocarbons: an environmental perspectives Atlas,R.M.
  8. Appl. Environ. Microbiol. v.31 The effect of iron on the biodegradation of petroleum in sea water Bartha,R.;J.T.Dibble
  9. Adv. Appl. Microbiol. v.22 The microbiology of aquatic oil spills Bartha,R.;R.M.Atlas
  10. Petroleum microbiology The fate of petroleum in soil ecosystems Bossert,I.;R.Bartha;M.Atlas(ed.)
  11. J. Fish. Res. Board Can. v.35 Microbial ecology studies of the Metula spill in the Strait of Magellan Colwell,R.R.;A.L.Mills;J.D.Walker;P.Garcia-Tello;V.Campos-P.
  12. Petroleum microbiology The fate of petroleum pollutants in freshwater ecosystems Cooney,J.J.;M.Atlas(ed.)
  13. Microb. Ecol. v.11 Factors influencing hydrocarbon degradation in the freshwater lakes Cooney,J.J.;S.A.Silver;E.A.Beck
  14. Appl. Environ. Microbiol. v.37 Effect of environmental parameters on the biodegradation of oil sludge Dibble,J.T.;R.Bartha
  15. W. Erg. der Schwed. S$\"{u}$dpolar Expedition v.4 Bakteriologische Studien mit Rand der schwedischen Sudpolar Expedition 1901-1903 Ekelof,E.
  16. Petroleum microbiology The fate of petroleum in marine ecosystems Floodgate,G.;M.Atlas(ed.)
  17. Proceedings of Joint Conference on Prevention and Control of Oil Spills Experimental ecosystems to measure fate of Oil Spills dispersed by surface active products Gatelier,C.R.;J.L.Oudin;P.Fusey;J.C.Lacase;M.L.Priou
  18. Microb. Ecol. v.10 Observations of microbial percent respiration values in arctic and subarctic marine waters and sediments Griffiths,R.P.;B.A.Caldwell;R.Y.Morita
  19. Helgol. Wiss. Meeresunters. v.15 Experimentellokologische Untersuchungen ber die limitierenden Faktoren des mikrobiellen labbaucs in marinen Milieu Gunkel,W.
  20. Nature(London) v.215 Thermophilic bacteria isolated on n-tetradecane Klug,M.J.;A.J.Markovitz
  21. Bacteriol. Rev. v.39 Psychrophilic bacteria Morita,R.Y.
  22. Appl. Microbiol. v.28 Effect of environmental parameters on bacterial degradation of Bunker C oil, crude oils and hydrocarbons Mulkins-Philips,G.J.;J.E.Stewart
  23. Appl. Environ. Microbiol. v.31 Microbial degradation of oil spills enhanced by a slow-release fertilizer Olivieri,R.;P.Bacchin;A.Robertiello;N.Oddo;L.Degen;A.Tonolo
  24. J. Bacteriol. v.91 Repair of thermal injury of Staphylococcus aureus Ordal,Z.J.;J.J.Iandolo
  25. Appl. Microbiol. v.24 Microbial degradation of oil: factors affecting the dispersion in sea water by mixed and pure cultures Reisfield,A.;E.Rosenberg;D.Gutnik
  26. Microbiologie der gewasser(3rd ed.) Rheinheimer,G.
  27. Recent progress in microbiology General biology and nomenclature of psychrophilic bacteria Stokes,J.L.;N.E.Gibbons(ed.)
  28. The microbial degradation of oil pollutants, Publication no. LSUSG73-01 Bacterial degradation of petroleum materials in low temperature marine environments Traxler,R.W.;D.G.Ahearn(ed.);S.P.Meyers(ed.)
  29. Microb. Ecol. v.1 Microbial degradation of model petroleum at low temperatures Walker,J.D.;R.R.Colwell
  30. Appl. Environ. Microbiol. v.31 Environmental factors influencing the rate of hydrocarbon oxidation in temperate lakes Ward,D.M.;T.D.Brock
  31. Can. J. Microbiol. v.20 Biodegradability and crude oil composition Westlake,D.W.S.;A.Jobson;R.Phillippe;F.D.Cook
  32. Adv. Water Poll. Res. v.3 The occurrence, effects and fate of oil polluting the sea ZoBell,C.E.
  33. Proceedings of the Joint Conferences on Prevention and Control of Oil Spills Microbial modification of oil in the sea ZoBell,C.E.
  34. Bacteriol. Rev. v.10 Action of microorganisms on hydrocarbons ZoBell,C.E.