대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제14권1호
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  • Pages.51-62
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  • 1993
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  • 1229-0807(pISSN)
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  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지

수축된 콜라겐 격자와 배양된 각질형성세포를 이용한 피부 대용물질의 제조에 관한 연구

Preparation of Living Skin Equivalent by using the Contracted Collagen Lattice and Cultured Human Keratinocytes

  • 박재경 (경희대학교 의과대학 부속병원 면역학연구실) ;
  • 조금철 (경희대학교 의과대학 부속병원 외과학교실) ;
  • 박호철 (경희대학교 의과대학 부속병원 외과학교실)
  • Park, Jae-Gyeong (Laboratory of Immunology, Kyung Hee University) ;
  • Jo, Geum-Cheol (Department of General Surgery, Kyung Hee University Hospital) ;
  • Park, Ho-Cheol (Department of General Surgery, Kyung Hee University Hospital)
  • 발행 : 1993.03.01
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초록

An experimental study was performed for the preparation of living skin-equivalent by the using collagen gel contraction with human fibroblasts as neodermls and cultured human keratinocytes as neoderm is . The results were as follows ; 1) The rate of collagen gel contraction was dependent on the number of fibroblasts into the lattice and collagen contraction was progressed according to the increment of the number of the cells. 2) The rate of collagen gel contraction was progressed according to the decrement of the contraction of the collagen. 3) The rate of gel contraction was progressed according to the increment of serum concentration in the fixed concentration of the fibroblasts and collagen. 4) The lattice contraction was decreased according to the increment of the population doublings of the fibroblasts. 5) Macroscopically, the artificial dermis was gray white in color and tissue-like consistency and elas- ticity. 6) Microscopically, three dimensionally contracted artificial dermis showed more dense fibroblasts and its newly formed collagen fibrils in the matrix than one dimensionally contracted one. 7) Finally prepared skin-equivalent showed good attachment of living stratified keratinocytes to the dermal equivalent microscopically. It has been proposed that newly formed skin-equivalent is suitable for the graft of extensively and deeply burned patients. Shortening of the manufacturing period of skin-equivalent and development of conservation technique as a readily usable state are to be solved for our ongoing works.

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참고문헌

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